Control of cell polarity and chemotaxis by Akt/PKB and PI3 kinase through the regulation of PAKa.
Published
Journal Article
We demonstrate that PI3 kinase and protein kinase B (PKB or Akt) control cell polarity and chemotaxis, in part, through the regulation of PAKa, which is required for myosin II assembly. We demonstrate that PI3K and PKB mediate PAKa's subcellular localization, PAKa's activation in response to chemoattractant stimulation, and chemoattractant-mediated myosin II assembly. Mutation of the PKB phosphorylation site in PAKa to Ala blocks PAKa's activation and inhibits PAKa redistribution in response to chemoattractant stimulation, whereas an Asp substitution leads to an activated protein. Addition of the PI3K inhibitor LY294002 results in a rapid loss of cell polarity and the axial distribution of actin, myosin, and PAKa. These results provide a mechanism by which PI3K regulates chemotaxis.
Full Text
Duke Authors
Cited Authors
- Chung, CY; Potikyan, G; Firtel, RA
Published Date
- May 2001
Published In
Volume / Issue
- 7 / 5
Start / End Page
- 937 - 947
PubMed ID
- 11389841
Pubmed Central ID
- 11389841
Electronic International Standard Serial Number (EISSN)
- 1097-4164
International Standard Serial Number (ISSN)
- 1097-2765
Digital Object Identifier (DOI)
- 10.1016/s1097-2765(01)00247-7
Language
- eng