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Combinatorial Single-Cell Analyses of Granulocyte-Monocyte Progenitor Heterogeneity Reveals an Early Uni-potent Neutrophil Progenitor.

Publication ,  Journal Article
Kwok, I; Becht, E; Xia, Y; Ng, M; Teh, YC; Tan, L; Evrard, M; Li, JLY; Tran, HTN; Tan, Y; Liu, D; Mishra, A; Liong, KH; Leong, K; Zhang, Y ...
Published in: Immunity
August 18, 2020

Granulocyte-monocyte progenitors (GMPs) have been previously defined for their potential to generate various myeloid progenies such as neutrophils and monocytes. Although studies have proposed lineage heterogeneity within GMPs, it is unclear if committed progenitors already exist among these progenitors and how they may behave differently during inflammation. By combining single-cell transcriptomic and proteomic analyses, we identified the early committed progenitor within the GMPs responsible for the strict production of neutrophils, which we designate as proNeu1. Our dissection of the GMP hierarchy led us to further identify a previously unknown intermediate proNeu2 population. Similar populations could be detected in human samples. proNeu1s, but not proNeu2s, selectively expanded during the early phase of sepsis at the expense of monocytes. Collectively, our findings help shape the neutrophil maturation trajectory roadmap and challenge the current definition of GMPs.

Duke Scholars

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Published In

Immunity

DOI

EISSN

1097-4180

Publication Date

August 18, 2020

Volume

53

Issue

2

Start / End Page

303 / 318.e5

Location

United States

Related Subject Headings

  • Single-Cell Analysis
  • Neutrophils
  • Myelopoiesis
  • Monocytes
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Immunology
  • Humans
  • Granulocyte Precursor Cells
 

Citation

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Kwok, I., Becht, E., Xia, Y., Ng, M., Teh, Y. C., Tan, L., … Ng, L. G. (2020). Combinatorial Single-Cell Analyses of Granulocyte-Monocyte Progenitor Heterogeneity Reveals an Early Uni-potent Neutrophil Progenitor. Immunity, 53(2), 303-318.e5. https://doi.org/10.1016/j.immuni.2020.06.005
Kwok, Immanuel, Etienne Becht, Yu Xia, Melissa Ng, Ye Chean Teh, Leonard Tan, Maximilien Evrard, et al. “Combinatorial Single-Cell Analyses of Granulocyte-Monocyte Progenitor Heterogeneity Reveals an Early Uni-potent Neutrophil Progenitor.Immunity 53, no. 2 (August 18, 2020): 303-318.e5. https://doi.org/10.1016/j.immuni.2020.06.005.
Kwok I, Becht E, Xia Y, Ng M, Teh YC, Tan L, et al. Combinatorial Single-Cell Analyses of Granulocyte-Monocyte Progenitor Heterogeneity Reveals an Early Uni-potent Neutrophil Progenitor. Immunity. 2020 Aug 18;53(2):303-318.e5.
Kwok, Immanuel, et al. “Combinatorial Single-Cell Analyses of Granulocyte-Monocyte Progenitor Heterogeneity Reveals an Early Uni-potent Neutrophil Progenitor.Immunity, vol. 53, no. 2, Aug. 2020, pp. 303-318.e5. Pubmed, doi:10.1016/j.immuni.2020.06.005.
Kwok I, Becht E, Xia Y, Ng M, Teh YC, Tan L, Evrard M, Li JLY, Tran HTN, Tan Y, Liu D, Mishra A, Liong KH, Leong K, Zhang Y, Olsson A, Mantri CK, Shyamsunder P, Liu Z, Piot C, Dutertre C-A, Cheng H, Bari S, Ang N, Biswas SK, Koeffler HP, Tey HL, Larbi A, Su I-H, Lee B, St John A, Chan JKY, Hwang WYK, Chen J, Salomonis N, Chong SZ, Grimes HL, Liu B, Hidalgo A, Newell EW, Cheng T, Ginhoux F, Ng LG. Combinatorial Single-Cell Analyses of Granulocyte-Monocyte Progenitor Heterogeneity Reveals an Early Uni-potent Neutrophil Progenitor. Immunity. 2020 Aug 18;53(2):303-318.e5.
Journal cover image

Published In

Immunity

DOI

EISSN

1097-4180

Publication Date

August 18, 2020

Volume

53

Issue

2

Start / End Page

303 / 318.e5

Location

United States

Related Subject Headings

  • Single-Cell Analysis
  • Neutrophils
  • Myelopoiesis
  • Monocytes
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Immunology
  • Humans
  • Granulocyte Precursor Cells