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Cell Line Authentication in Vision Research and Beyond: A Tale Retold.

Publication ,  Journal Article
Peavey, J; Malek, G
Published in: Invest Ophthalmol Vis Sci
June 3, 2020

We live in an age where new technologies, and organizations involved in the distribution of biological materials, such as cell culture lines, have eased accessibility to a variety of in vitro models, developed, and/or harvested from different sources. In translational and basic ophthalmology research, in vitro assays are an essential component to discovery and preclinical studies. It is, therefore, of utmost importance for vision researchers to be cognizant of the risks surrounding the use of newly developed cell culture models and how scientific integrity could be impacted when standard operating procedures are not followed for cell line validation and identification. Herein, we discuss authentication challenges we faced when we obtained a newly marketed human choroidal endothelial cell line for vision research, and outline our process of validating and characterizing primary human choroidal endothelial cell lines in the laboratory.

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Published In

Invest Ophthalmol Vis Sci

DOI

EISSN

1552-5783

Publication Date

June 3, 2020

Volume

61

Issue

6

Start / End Page

19

Location

United States

Related Subject Headings

  • Real-Time Polymerase Chain Reaction
  • Ophthalmology & Optometry
  • Ophthalmology
  • Humans
  • Genetic Loci
  • Flow Cytometry
  • Endothelial Cells
  • Choroid
  • Cell Line Authentication
  • Cell Line
 

Citation

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Peavey, J., & Malek, G. (2020). Cell Line Authentication in Vision Research and Beyond: A Tale Retold. Invest Ophthalmol Vis Sci, 61(6), 19. https://doi.org/10.1167/iovs.61.6.19
Peavey, Jeremy, and Goldis Malek. “Cell Line Authentication in Vision Research and Beyond: A Tale Retold.Invest Ophthalmol Vis Sci 61, no. 6 (June 3, 2020): 19. https://doi.org/10.1167/iovs.61.6.19.
Peavey J, Malek G. Cell Line Authentication in Vision Research and Beyond: A Tale Retold. Invest Ophthalmol Vis Sci. 2020 Jun 3;61(6):19.
Peavey, Jeremy, and Goldis Malek. “Cell Line Authentication in Vision Research and Beyond: A Tale Retold.Invest Ophthalmol Vis Sci, vol. 61, no. 6, June 2020, p. 19. Pubmed, doi:10.1167/iovs.61.6.19.
Peavey J, Malek G. Cell Line Authentication in Vision Research and Beyond: A Tale Retold. Invest Ophthalmol Vis Sci. 2020 Jun 3;61(6):19.

Published In

Invest Ophthalmol Vis Sci

DOI

EISSN

1552-5783

Publication Date

June 3, 2020

Volume

61

Issue

6

Start / End Page

19

Location

United States

Related Subject Headings

  • Real-Time Polymerase Chain Reaction
  • Ophthalmology & Optometry
  • Ophthalmology
  • Humans
  • Genetic Loci
  • Flow Cytometry
  • Endothelial Cells
  • Choroid
  • Cell Line Authentication
  • Cell Line