UbcH5 Interacts with Substrates to Participate in Lysine Selection with the E3 Ubiquitin Ligase CHIP.
The E3 ubiquitin ligase C-terminus of Hsc70 interacting protein (CHIP) plays a critical role in regulating the ubiquitin-dependent degradation of misfolded proteins. CHIP mediates the ubiquitination of the α-amino-terminus of substrates with the E2 Ube2w and facilitates the ubiquitination of lysine residues with the E2 UbcH5. While it is known that Ube2w directly interacts with the disordered regions at the N-terminus of its substrates, it is unclear how CHIP and UbcH5 mediate substrate lysine selection. Here, we have decoupled the contributions of the E2, UbcH5, and the E3, CHIP, in ubiquitin transfer. We show that UbcH5 selects substrate lysine residues independent of CHIP, and that CHIP participates in lysine selection by fine-tuning the subset of substrate lysines that are ubiquitinated. We also identify lysine 128 near the C-terminus of UbcH5 as a critical residue for the efficient ubiquitin transfer by UbcH5 in both the presence and absence of CHIP. Together, these data demonstrate an important role of the UbcH5/substrate interactions in mediating the efficient ubiquitin transfer by the CHIP/UbcH5 complex.
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Related Subject Headings
- Ubiquitination
- Ubiquitin-Protein Ligases
- Ubiquitin-Conjugating Enzymes
- Models, Molecular
- Lysine
- Humans
- Biochemistry & Molecular Biology
- 3404 Medicinal and biomolecular chemistry
- 3205 Medical biochemistry and metabolomics
- 3101 Biochemistry and cell biology
Citation
Published In
DOI
EISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Ubiquitination
- Ubiquitin-Protein Ligases
- Ubiquitin-Conjugating Enzymes
- Models, Molecular
- Lysine
- Humans
- Biochemistry & Molecular Biology
- 3404 Medicinal and biomolecular chemistry
- 3205 Medical biochemistry and metabolomics
- 3101 Biochemistry and cell biology