Curcumin inhibits LPS-induced CCL2 expression via JNK pathway in C6 rat astrocytoma cells.

Published

Journal Article

The important role of neuroinflammation in many chronic and acute pathological conditions of the central nervous system is widely recognized. Curcumin is a major component of turmeric and reportedly has anti-inflammatory and anti-oxidant effects. This study investigated the inhibitory effect of curcumin on lipopolysacharide (LPS)-induced chemokine CCL2 (or monocyte chemoattractant protein-1, MCP-1) production and whether the effect is mediated by mitogen-activated protein kinases (MAPKs) in the rat astrocytoma cell C6. We observed that LPS (1 μg/ml) induced the upregulation of CCL2 mRNA and protein in C6. Treatment with curcumin (2.5, 10, and 25 μM) decreased the expression of CCL2 mRNA and protein in a dose-dependent manner under treatment with LPS. Additionally, the c-jun N-terminal kinase (JNK) inhibitor (SP600125) dose-dependently inhibited LPS-induced CCL2 upregulation, whereas the MAPK kinase (MEK) inhibitor (PD98059) only had a mild effect and the p38 MAPK inhibitor (SB203580) had no effect. Finally, western blot showed that LPS induced rapid JNK activation and curcumin reduced LPS-induced phosphoJNK (pJNK) expression at 30 min after LPS stimulation. These data suggest that the anti-neuroinflammatory effect of curcumin relates to the downregulation of CCL2 expression through the JNK pathway in astrocytoma cells, which indicates a possible benefit from the use of curcumin in the treatment of neuroinflammation-associated disorders.

Full Text

Duke Authors

Cited Authors

  • Zhang, Z-J; Zhao, L-X; Cao, D-L; Zhang, X; Gao, Y-J; Xia, C

Published Date

  • August 2012

Published In

Volume / Issue

  • 32 / 6

Start / End Page

  • 1003 - 1010

PubMed ID

  • 22410671

Pubmed Central ID

  • 22410671

Electronic International Standard Serial Number (EISSN)

  • 1573-6830

Digital Object Identifier (DOI)

  • 10.1007/s10571-012-9816-4

Language

  • eng

Conference Location

  • United States