Brain-derived neurotrophic factor-activated astrocytes produce mechanical allodynia in neuropathic pain.

Published

Journal Article

Neuropathic pain management is challenging for physicians and a vexing problem for basic researchers. Recent studies reveal that activated spinal astrocytes may play a vital role in nerve injury-induced neuropathic pain, although the mechanisms are not fully understood. We have found increased glial fibrillary acidic protein (GFAP) expression, a hallmark of reactive gliosis, and elevated brain-derived neurotrophic factor (BDNF) expression in the dorsal horn in a rat model of allodynia induced by spinal nerve ligation (SNL). The high GFAP expression and mechanical allodynia that SNL induces were prevented by the intrathecal injection of the BDNF-sequestering fusion protein TrkB/Fc. Additionally, mechanical allodynia and GFAP overexpression was induced by the spinal administration of exogenous BDNF to naive rats, and exogenous BDNF given together with fluorocitrate, an astrocytic metabolism inhibitor, inhibited allodynia and GFAP upregulation. Exogenous BDNF also activated the astrocytes directly when tested in vitro. Furthermore, intrathecal administration of BDNF-stimulated astrocytes also induced mechanical allodynia in naive rats. All of these results indicate that astrocytes activated by BDNF might contribute to mechanical allodynia development in neuropathic pain in rats.

Full Text

Duke Authors

Cited Authors

  • Zhang, X; Wang, J; Zhou, Q; Xu, Y; Pu, S; Wu, J; Xue, Y; Tian, Y; Lu, J; Jiang, W; Du, D

Published Date

  • December 29, 2011

Published In

Volume / Issue

  • 199 /

Start / End Page

  • 452 - 460

PubMed ID

  • 22044922

Pubmed Central ID

  • 22044922

Electronic International Standard Serial Number (EISSN)

  • 1873-7544

Digital Object Identifier (DOI)

  • 10.1016/j.neuroscience.2011.10.017

Language

  • eng

Conference Location

  • United States