The interaction of biological and noxious transition metals with the zinc probes FluoZin-3 and Newport Green.

Journal Article (Journal Article)

Zinc-sensitive fluorescent probes have become increasingly important in the investigation of the cellular roles of zinc. There is, however, little information on how the other transition metals in cells may influence the measurement of zinc. We have characterized in vitro the interaction of the nominal zinc indicators FluoZin-3 and Newport Green with all the cationic transition metals found within cells, Cr, Mn, Fe, Co, and Cu, as well as Ni and Cd, by measuring their dissociation constants. In addition, we have shown how FluoZin-3 can be used to quantify the concentration of copper in a cell-free assay and report that the fluorescence of Newport Green is boosted by both Cu(I) and Fe(II). Furthermore, we have introduced diagnostics for detecting the interference of metals other than zinc with its measurement within cells.

Full Text

Duke Authors

Cited Authors

  • Zhao, J; Bertoglio, BA; Devinney, MJ; Dineley, KE; Kay, AR

Published Date

  • January 1, 2009

Published In

Volume / Issue

  • 384 / 1

Start / End Page

  • 34 - 41

PubMed ID

  • 18848515

Pubmed Central ID

  • PMC2630397

Electronic International Standard Serial Number (EISSN)

  • 1096-0309

Digital Object Identifier (DOI)

  • 10.1016/j.ab.2008.09.019


  • eng

Conference Location

  • United States