Enrichment of malondialdehyde-acetaldehyde antibody in the rheumatoid arthritis joint.

Journal Article (Journal Article)


To characterize the expression of malondialdehdye-acetaldehyde (MAA) adducts and anti-MAA antibody in articular tissues and serum of patients with RA.


Paired sera and SF were examined from 29 RA and 13 OA patients. Anti-MAA antibody, RF, ACPA and total immunoglobulin were quantified. SF-serum measures were compared within and between disease groups. The presence and co-localization of MAA, citrulline and select leukocyte antigens in RA and OA synovial tissues were examined using immunohistochemistry.


Circulating and SF anti-MAA antibody concentrations were higher in RA vs OA by 1.5- to 5-fold. IgG (P < 0.001), IgM (P = 0.006) and IgA (P = 0.036) anti-MAA antibodies were higher in paired RA SF than serum, differences not observed for total immunoglobulin, RF or ACPA. In RA synovial tissues, co-localization of MAA with citrulline and CD19+ or CD27+ B cells was demonstrated and was much higher in magnitude than MAA or citrulline co-localization with T cells, monocytes, macrophages or dendritic cells (P < 0.01).


Anti-MAA antibodies are present in higher concentrations in the RA joint compared with sera, a finding not observed for other disease-related autoantibodies. Co-localization of MAA and citrulline with mature B cells, coupled with the local enrichment of anti-MAA immune responses, implicates MAA-adduct formation in local autoantibody production.

Full Text

Duke Authors

Cited Authors

  • Mikuls, TR; Duryee, MJ; Rahman, R; Anderson, DR; Sayles, HR; Hollins, A; Michaud, K; Wolfe, F; Thiele, GE; Sokolove, J; Robinson, WH; Lingampalli, N; Nicholas, AP; Talmon, GA; Su, K; Zimmerman, MC; Klassen, LW; Thiele, GM

Published Date

  • October 2017

Published In

Volume / Issue

  • 56 / 10

Start / End Page

  • 1794 - 1803

PubMed ID

  • 28957552

Pubmed Central ID

  • PMC5850833

Electronic International Standard Serial Number (EISSN)

  • 1462-0332

International Standard Serial Number (ISSN)

  • 1462-0324

Digital Object Identifier (DOI)

  • 10.1093/rheumatology/kex212


  • eng