Smoothened inhibition leads to decreased proliferation and induces apoptosis in esophageal adenocarcinoma cells.
Journal Article (Journal Article)
The Hedgehog (Hh) pathway is known to be active in Barrett's carcinogenesis. Therefore, we evaluated the efficacy and underlying mechanisms of inhibition of cancer cell growth by the smoothened (Smo) antagonist BMS-833923 in esophageal adenocarcinoma (EAC) cell lines. Cell proliferation and apoptosis were evaluated by flow cytometry, Western blotting, immunofluorescence, and quantitative reverse transcription polymerase chain reactions. Results showed that the Smo antagonist led to reduced Hh pathway activity, resulting in decreased cell proliferation and induction of apoptosis via the intrinsic pathway in the esophageal cancer cells. In conclusion, the Smo antagonist may have application as an EAC chemotherapeutic agent.
Full Text
Duke Authors
Cited Authors
- Zaidi, AH; Komatsu, Y; Kelly, LA; Malhotra, U; Rotoloni, C; Kosovec, JE; Zahoor, H; Makielski, R; Bhatt, A; Hoppo, T; Jobe, BA
Published Date
- August 2013
Published In
Volume / Issue
- 31 / 7
Start / End Page
- 480 - 489
PubMed ID
- 23915072
Electronic International Standard Serial Number (EISSN)
- 1532-4192
International Standard Serial Number (ISSN)
- 0735-7907
Digital Object Identifier (DOI)
- 10.3109/07357907.2013.820317
Language
- eng