Smoothened inhibition leads to decreased proliferation and induces apoptosis in esophageal adenocarcinoma cells.

Journal Article (Journal Article)

The Hedgehog (Hh) pathway is known to be active in Barrett's carcinogenesis. Therefore, we evaluated the efficacy and underlying mechanisms of inhibition of cancer cell growth by the smoothened (Smo) antagonist BMS-833923 in esophageal adenocarcinoma (EAC) cell lines. Cell proliferation and apoptosis were evaluated by flow cytometry, Western blotting, immunofluorescence, and quantitative reverse transcription polymerase chain reactions. Results showed that the Smo antagonist led to reduced Hh pathway activity, resulting in decreased cell proliferation and induction of apoptosis via the intrinsic pathway in the esophageal cancer cells. In conclusion, the Smo antagonist may have application as an EAC chemotherapeutic agent.

Full Text

Duke Authors

Kosovec, Juliann

Cited Authors

Zaidi, AH; Komatsu, Y; Kelly, LA; Malhotra, U; Rotoloni, C; Kosovec, JE; Zahoor, H; Makielski, R; Bhatt, A; Hoppo, T; Jobe, BA

Published Date

August 2013

Published In

Cancer Investigation

Volume / Issue

31 / 7

Start / End Page

480 - 489

PubMed ID

23915072

Pubmed Central ID

23915072

Electronic International Standard Serial Number (EISSN)

1532-4192

International Standard Serial Number (ISSN)

0735-7907

Digital Object Identifier (DOI)

10.3109/07357907.2013.820317

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