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Smoothened inhibition leads to decreased proliferation and induces apoptosis in esophageal adenocarcinoma cells.

Publication ,  Journal Article
Zaidi, AH; Komatsu, Y; Kelly, LA; Malhotra, U; Rotoloni, C; Kosovec, JE; Zahoor, H; Makielski, R; Bhatt, A; Hoppo, T; Jobe, BA
Published in: Cancer investigation
August 2013

The Hedgehog (Hh) pathway is known to be active in Barrett's carcinogenesis. Therefore, we evaluated the efficacy and underlying mechanisms of inhibition of cancer cell growth by the smoothened (Smo) antagonist BMS-833923 in esophageal adenocarcinoma (EAC) cell lines. Cell proliferation and apoptosis were evaluated by flow cytometry, Western blotting, immunofluorescence, and quantitative reverse transcription polymerase chain reactions. Results showed that the Smo antagonist led to reduced Hh pathway activity, resulting in decreased cell proliferation and induction of apoptosis via the intrinsic pathway in the esophageal cancer cells. In conclusion, the Smo antagonist may have application as an EAC chemotherapeutic agent.

Duke Scholars

Published In

Cancer investigation

DOI

EISSN

1532-4192

ISSN

0735-7907

Publication Date

August 2013

Volume

31

Issue

7

Start / End Page

480 / 489

Related Subject Headings

  • Smoothened Receptor
  • Signal Transduction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Receptors, G-Protein-Coupled
  • Oncology & Carcinogenesis
  • Humans
  • Hedgehog Proteins
  • Fluorescent Antibody Technique
  • Flow Cytometry
  • Esophageal Neoplasms
 

Citation

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Zaidi, A. H., Komatsu, Y., Kelly, L. A., Malhotra, U., Rotoloni, C., Kosovec, J. E., … Jobe, B. A. (2013). Smoothened inhibition leads to decreased proliferation and induces apoptosis in esophageal adenocarcinoma cells. Cancer Investigation, 31(7), 480–489. https://doi.org/10.3109/07357907.2013.820317
Zaidi, Ali H., Yoshihiro Komatsu, Lori A. Kelly, Usha Malhotra, Christina Rotoloni, Juliann E. Kosovec, Haris Zahoor, et al. “Smoothened inhibition leads to decreased proliferation and induces apoptosis in esophageal adenocarcinoma cells.Cancer Investigation 31, no. 7 (August 2013): 480–89. https://doi.org/10.3109/07357907.2013.820317.
Zaidi AH, Komatsu Y, Kelly LA, Malhotra U, Rotoloni C, Kosovec JE, et al. Smoothened inhibition leads to decreased proliferation and induces apoptosis in esophageal adenocarcinoma cells. Cancer investigation. 2013 Aug;31(7):480–9.
Zaidi, Ali H., et al. “Smoothened inhibition leads to decreased proliferation and induces apoptosis in esophageal adenocarcinoma cells.Cancer Investigation, vol. 31, no. 7, Aug. 2013, pp. 480–89. Epmc, doi:10.3109/07357907.2013.820317.
Zaidi AH, Komatsu Y, Kelly LA, Malhotra U, Rotoloni C, Kosovec JE, Zahoor H, Makielski R, Bhatt A, Hoppo T, Jobe BA. Smoothened inhibition leads to decreased proliferation and induces apoptosis in esophageal adenocarcinoma cells. Cancer investigation. 2013 Aug;31(7):480–489.

Published In

Cancer investigation

DOI

EISSN

1532-4192

ISSN

0735-7907

Publication Date

August 2013

Volume

31

Issue

7

Start / End Page

480 / 489

Related Subject Headings

  • Smoothened Receptor
  • Signal Transduction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Receptors, G-Protein-Coupled
  • Oncology & Carcinogenesis
  • Humans
  • Hedgehog Proteins
  • Fluorescent Antibody Technique
  • Flow Cytometry
  • Esophageal Neoplasms