Therapy with Cardiomyocytes Derived from Pluripotent Cells in Chronic Chagasic Cardiomyopathy.

Journal Article (Journal Article)

Chagas disease discovered more than a century ago remains an incurable disease. The objective of this work was to investigate the therapeutic potential of cardiomyocytes derived from mouse embryonic stem cells (CM-mESC) in a model of chronic Chagasic cardiomyopathy (CCC). Mouse embryonic stem cells (mESC) were characterized, transduced with luciferase, and submitted to cardiac differentiation. CM-mESC were labeled with superparamagnetic iron oxide particles. To induce CCC, mice were infected with Brazil strain trypomastigotes. At 150 days post-infection (dpi), infected animals were treated with CM-mESC or PBS. Cells were detected by magnetic resonance imaging (MRI) and bioluminescence. Cardiac function was evaluated by MRI and electrocardiogram at 150 and 196 dpi. CCC mice showed significant differences in MRI and ECG parameters compared to non-infected mice. However, no differences were observed in contractile and electrical parameters between cell and PBS injected groups, 45 days after cell transplantation. Cells were detected 24 h after transplantation by MRI. CM-mESC bioluminescence tracking demonstrated over 90% decrease in signal 8 days after treatment. Nevertheless, the Infected + CM-mESC group showed a significant reduction in the percentage of collagen fibers when compared to the Infected + PBS group. In conclusion, CM-mESC therapy was not effective in reversing cardiac functional changes induced by Chagas disease despite some improvement in myocardial fibrosis.

Full Text

Duke Authors

Cited Authors

  • Brasil, GV; Silva Dos Santos, D; Mendonça, EA; Mesquita, FCP; Kasai-Brunswick, TH; Cunha, STD; Pimentel, CF; Vasconcelos-Dos-Santos, AD; Mendez-Otero, R; Azevedo Filho, CFD; Goldenberg, RCDS; Campos de Carvalho, AC

Published Date

  • July 7, 2020

Published In

Volume / Issue

  • 9 / 7

PubMed ID

  • 32645832

Pubmed Central ID

  • PMC7408395

Electronic International Standard Serial Number (EISSN)

  • 2073-4409

Digital Object Identifier (DOI)

  • 10.3390/cells9071629

Language

  • eng

Conference Location

  • Switzerland