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Comparison of the platelet activation status of single-donor platelets obtained with two different cell separator technologies.

Publication ,  Journal Article
Millar, D; Hayes, C; Jones, J; Klapper, E; Kniep, JN; Luu, HS; Noland, DK; Petitti, L; Poisson, JL; Spaepen, E; Ye, Z; Maurer-Spurej, E
Published in: Transfusion
September 2020

BACKGROUND: The microparticle content (MP%) of apheresis platelets-a marker of platelet activation-is influenced by donor factors and by external stressors during collection and storage. This study assessed the impact of apheresis technology and other factors on the activation status (MP%) of single-donor apheresis platelets. STUDY DESIGN AND METHODS: Data from six US hospitals that screened platelets by measuring MP% through dynamic light scattering (ThromboLUX) were retrospectively analyzed. Relative risks (RRs) were derived from univariate and multivariable regression models, with activation rate (MP% ≥15% for plasma-stored platelets; ≥10% for platelet additive solution [PAS]-stored platelets) and MP% as outcomes. Apheresis platform (Trima Accel vs Amicus), storage medium (plasma vs PAS), pathogen reduction, storage time, and testing location were used as predictors. RESULTS: Data were obtained from 7511 platelet units collected using Trima (from 16 suppliers, all stored in plasma, 20.0% were pathogen-reduced) and 2456 collected using Amicus (from four different collection facilities of one supplier, 65.0% plasma-stored, 35.0% PAS-stored, none pathogen-reduced). Overall, 30.0% of Trima platelets were activated compared to 45.6% of Amicus platelets (P < .0001). Multivariable analysis identified apheresis platform as significantly associated with platelet activation, with a lower activation rate for Trima than Amicus (RR: 0.641, 95% confidence interval [CI]: 0.578; 0.711, P < .0001) and a 6.901% (95% CI: 5.926; 7.876, P < .0001) absolute reduction in MP%, when adjusting for the other variables. CONCLUSION: Trima-collected platelets were significantly less likely to be activated than Amicus-collected platelets, irrespective of the storage medium, the use of pathogen reduction, storage time, and testing site.

Duke Scholars

Published In

Transfusion

DOI

EISSN

1537-2995

Publication Date

September 2020

Volume

60

Issue

9

Start / End Page

2067 / 2078

Location

United States

Related Subject Headings

  • Retrospective Studies
  • Plateletpheresis
  • Platelet Activation
  • Male
  • Humans
  • Female
  • Cardiovascular System & Hematology
  • Blood Preservation
  • Blood Platelets
  • Blood Donors
 

Citation

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ICMJE
MLA
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Millar, D., Hayes, C., Jones, J., Klapper, E., Kniep, J. N., Luu, H. S., … Maurer-Spurej, E. (2020). Comparison of the platelet activation status of single-donor platelets obtained with two different cell separator technologies. Transfusion, 60(9), 2067–2078. https://doi.org/10.1111/trf.15934
Millar, Daniel, Chelsea Hayes, Jessica Jones, Ellen Klapper, Joel N. Kniep, Hung S. Luu, Daniel K. Noland, et al. “Comparison of the platelet activation status of single-donor platelets obtained with two different cell separator technologies.Transfusion 60, no. 9 (September 2020): 2067–78. https://doi.org/10.1111/trf.15934.
Millar D, Hayes C, Jones J, Klapper E, Kniep JN, Luu HS, et al. Comparison of the platelet activation status of single-donor platelets obtained with two different cell separator technologies. Transfusion. 2020 Sep;60(9):2067–78.
Millar, Daniel, et al. “Comparison of the platelet activation status of single-donor platelets obtained with two different cell separator technologies.Transfusion, vol. 60, no. 9, Sept. 2020, pp. 2067–78. Pubmed, doi:10.1111/trf.15934.
Millar D, Hayes C, Jones J, Klapper E, Kniep JN, Luu HS, Noland DK, Petitti L, Poisson JL, Spaepen E, Ye Z, Maurer-Spurej E. Comparison of the platelet activation status of single-donor platelets obtained with two different cell separator technologies. Transfusion. 2020 Sep;60(9):2067–2078.
Journal cover image

Published In

Transfusion

DOI

EISSN

1537-2995

Publication Date

September 2020

Volume

60

Issue

9

Start / End Page

2067 / 2078

Location

United States

Related Subject Headings

  • Retrospective Studies
  • Plateletpheresis
  • Platelet Activation
  • Male
  • Humans
  • Female
  • Cardiovascular System & Hematology
  • Blood Preservation
  • Blood Platelets
  • Blood Donors