Topoisomerase III-β is required for efficient replication of positive-sense RNA viruses.
Based on genome-scale loss-of-function screens we discovered that Topoisomerase III-β (TOP3B), a human topoisomerase that acts on DNA and RNA, is required for yellow fever virus and dengue virus-2 replication. Remarkably, we found that TOP3B is required for efficient replication of all positive-sense-single stranded RNA viruses tested, including SARS-CoV-2. While there are no drugs that specifically inhibit this topoisomerase, we posit that TOP3B is an attractive anti-viral target.
Prasanth, KR; Hirano, M; Fagg, WS; McAnarney, ET; Shan, C; Xie, X; Hage, A; Pietzsch, CA; Bukreyev, A; Rajsbaum, R; Shi, P-Y; Bedford, MT; Bradrick, SS; Menachery, V; Garcia-Blanco, MA
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