Topoisomerase III-β is required for efficient replication of positive-sense RNA viruses.

Journal Article (Journal Article)

Based on genome-scale loss-of-function screens we discovered that Topoisomerase III-β (TOP3B), a human topoisomerase that acts on DNA and RNA, is required for yellow fever virus and dengue virus-2 replication. Remarkably, we found that TOP3B is required for efficient replication of all positive-sense-single stranded RNA viruses tested, including SARS-CoV-2. While there are no drugs that specifically inhibit this topoisomerase, we posit that TOP3B is an attractive anti-viral target.

Full Text

Duke Authors

Cited Authors

  • Prasanth, KR; Hirano, M; Fagg, WS; McAnarney, ET; Shan, C; Xie, X; Hage, A; Pietzsch, CA; Bukreyev, A; Rajsbaum, R; Shi, P-Y; Bedford, MT; Bradrick, SS; Menachery, V; Garcia-Blanco, MA

Published Date

  • October 2020

Published In

Volume / Issue

  • 182 /

Start / End Page

  • 104874 -

PubMed ID

  • 32735900

Pubmed Central ID

  • PMC7386308

Electronic International Standard Serial Number (EISSN)

  • 1872-9096

Digital Object Identifier (DOI)

  • 10.1016/j.antiviral.2020.104874

Language

  • eng

Conference Location

  • Netherlands