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Identifying host microRNAs in bronchoalveolar lavage samples from lung transplant recipients infected with Aspergillus.

Publication ,  Journal Article
Gohir, W; Klement, W; Singer, LG; Palmer, SM; Mazzulli, T; Keshavjee, S; Husain, S
Published in: J Heart Lung Transplant
November 2020

BACKGROUND: MicroRNAs (miRNAs) are small non-coding RNAs of ∼22 nucleotides that play a crucial role in post-transcriptional regulation of gene expression. Dysregulation of miRNA expression has been shown during microbial infections. We sought to identify miRNAs that distinguish invasive aspergillosis (IA) from non-IA in lung transplant recipients (LTRs). METHODS: We used NanoString nCounter Human miRNA, version 3, panel to measure miRNAs in bronchoalveolar lavage (BAL) samples from LTRs with Aspergillus colonization (ASP group) (n = 10), those with Aspergillus colonization and chronic lung allograft dysfunction (CLAD) (ASPCLAD group) (n = 7), those with IA without CLAD (IA group) (n = 10), those who developed IA with CLAD (IACLAD group) (n = 9), and control patients (controls) (n = 9). The miRNA profile was compared using the permutation test of 100,000 trials for each of the comparisons. We used mirDIP to obtain their gene targets and pathDIP to determine the pathway enrichment. RESULTS: We performed pairwise comparisons between patient groups to identify differentially expressed miRNAs. A total of 5 miRNAs were found to be specific to IA, including 4 (miR-145-5p, miR-424-5p, miR-99b-5p, and miR-4488) that were upregulated and the pair (miR-4454 + miR-7975) that was downregulated in IA group vs controls. The expression change for these miRNAs was specific to patients with IA; they were not significantly differentiated between IACLAD and IA groups. Signaling pathways associated with an immunologic response to IA were found to be significantly enriched. CONCLUSIONS: We report a set of 5 differentially expressed miRNAs in the BAL of LTRs with IA that might help in the development of diagnostic and prognostic tools for IA in LTRs. However, further investigation is needed in a larger cohort to validate the findings.

Duke Scholars

Published In

J Heart Lung Transplant

DOI

EISSN

1557-3117

Publication Date

November 2020

Volume

39

Issue

11

Start / End Page

1228 / 1237

Location

United States

Related Subject Headings

  • Transplant Recipients
  • Surgery
  • Retrospective Studies
  • Pulmonary Aspergillosis
  • Prognosis
  • Primary Graft Dysfunction
  • Middle Aged
  • MicroRNAs
  • Male
  • Lung Transplantation
 

Citation

APA
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Gohir, W., Klement, W., Singer, L. G., Palmer, S. M., Mazzulli, T., Keshavjee, S., & Husain, S. (2020). Identifying host microRNAs in bronchoalveolar lavage samples from lung transplant recipients infected with Aspergillus. J Heart Lung Transplant, 39(11), 1228–1237. https://doi.org/10.1016/j.healun.2020.07.014
Gohir, Wajiha, William Klement, Lianne G. Singer, Scott M. Palmer, Tony Mazzulli, Shaf Keshavjee, and Shahid Husain. “Identifying host microRNAs in bronchoalveolar lavage samples from lung transplant recipients infected with Aspergillus.J Heart Lung Transplant 39, no. 11 (November 2020): 1228–37. https://doi.org/10.1016/j.healun.2020.07.014.
Gohir W, Klement W, Singer LG, Palmer SM, Mazzulli T, Keshavjee S, et al. Identifying host microRNAs in bronchoalveolar lavage samples from lung transplant recipients infected with Aspergillus. J Heart Lung Transplant. 2020 Nov;39(11):1228–37.
Gohir, Wajiha, et al. “Identifying host microRNAs in bronchoalveolar lavage samples from lung transplant recipients infected with Aspergillus.J Heart Lung Transplant, vol. 39, no. 11, Nov. 2020, pp. 1228–37. Pubmed, doi:10.1016/j.healun.2020.07.014.
Gohir W, Klement W, Singer LG, Palmer SM, Mazzulli T, Keshavjee S, Husain S. Identifying host microRNAs in bronchoalveolar lavage samples from lung transplant recipients infected with Aspergillus. J Heart Lung Transplant. 2020 Nov;39(11):1228–1237.
Journal cover image

Published In

J Heart Lung Transplant

DOI

EISSN

1557-3117

Publication Date

November 2020

Volume

39

Issue

11

Start / End Page

1228 / 1237

Location

United States

Related Subject Headings

  • Transplant Recipients
  • Surgery
  • Retrospective Studies
  • Pulmonary Aspergillosis
  • Prognosis
  • Primary Graft Dysfunction
  • Middle Aged
  • MicroRNAs
  • Male
  • Lung Transplantation