Adoptive Transfer of Renal Allograft Tolerance in a Large Animal Model.

Journal Article

Our recent studies in an inbred swine model demonstrated that both peripheral and intra-graft regulatory cells were required for the adoptive transfer of tolerance to a second, naïve donor-matched kidney. Here, we have asked whether both peripheral and intra-graft regulatory elements are required for adoptive transfer of tolerance when only a long-term tolerant (LTT) kidney is transplanted. Nine highly-inbred swine underwent a tolerance-inducing regimen to prepare LTT kidney grafts which were then transplanted to histocompatible recipients, with or without the peripheral cell populations required for adoptive transfer of tolerance to a naïve kidney. In contrast to our previous studies, tolerance of the LTT kidney transplants alone was achieved without transfer of additional peripheral cells and without strategies to increase the number/potency of regulatory T cells in the donor. This tolerance was systemic, since most subsequent, donor-matched challenge kidney grafts were accepted. These results confirm the presence of a potent tolerance-inducing and/or tolerance-maintaining cell population within LTT renal allografts. They suggest further that additional peripheral tolerance mechanisms, required for adoptive transfer of tolerance to a naïve donor-matched kidney, depend on peripheral cells that, if not transferred with the LTT kidney, require time to develop in the adoptive host.

Full Text

Duke Authors

Cited Authors

  • Villani, V; Yamada, K; Scalea, JR; Gillon, BC; Arn, JS; Sekijima, M; Tasaki, M; Cormack, TA; Moran, SG; Torabi, R; Shimizu, A; Sachs, DH

Published Date

  • January 2016

Published In

Volume / Issue

  • 16 / 1

Start / End Page

  • 317 - 324

PubMed ID

  • 26260215

Pubmed Central ID

  • 26260215

Electronic International Standard Serial Number (EISSN)

  • 1600-6143

International Standard Serial Number (ISSN)

  • 1600-6135

Digital Object Identifier (DOI)

  • 10.1111/ajt.13424

Language

  • eng