Adoptive Transfer of Renal Allograft Tolerance in a Large Animal Model.
Journal Article
Our recent studies in an inbred swine model demonstrated that both peripheral and intra-graft regulatory cells were required for the adoptive transfer of tolerance to a second, naïve donor-matched kidney. Here, we have asked whether both peripheral and intra-graft regulatory elements are required for adoptive transfer of tolerance when only a long-term tolerant (LTT) kidney is transplanted. Nine highly-inbred swine underwent a tolerance-inducing regimen to prepare LTT kidney grafts which were then transplanted to histocompatible recipients, with or without the peripheral cell populations required for adoptive transfer of tolerance to a naïve kidney. In contrast to our previous studies, tolerance of the LTT kidney transplants alone was achieved without transfer of additional peripheral cells and without strategies to increase the number/potency of regulatory T cells in the donor. This tolerance was systemic, since most subsequent, donor-matched challenge kidney grafts were accepted. These results confirm the presence of a potent tolerance-inducing and/or tolerance-maintaining cell population within LTT renal allografts. They suggest further that additional peripheral tolerance mechanisms, required for adoptive transfer of tolerance to a naïve donor-matched kidney, depend on peripheral cells that, if not transferred with the LTT kidney, require time to develop in the adoptive host.
Full Text
Duke Authors
Cited Authors
- Villani, V; Yamada, K; Scalea, JR; Gillon, BC; Arn, JS; Sekijima, M; Tasaki, M; Cormack, TA; Moran, SG; Torabi, R; Shimizu, A; Sachs, DH
Published Date
- January 2016
Published In
Volume / Issue
- 16 / 1
Start / End Page
- 317 - 324
PubMed ID
- 26260215
Pubmed Central ID
- 26260215
Electronic International Standard Serial Number (EISSN)
- 1600-6143
International Standard Serial Number (ISSN)
- 1600-6135
Digital Object Identifier (DOI)
- 10.1111/ajt.13424
Language
- eng