Isolation of Microglia from Mouse or Human Tissue.

Journal Article (Journal Article)

Microglia are the innate immune cells of the central nervous system. Although numerous methods have been developed to isolate microglia from the brain, the method of dissociation and isolation can have a profound effect on the function of these highly dynamic cells. Here, we present an optimized protocol to isolate CD11b+ cells (microglia) from mouse or human brain tissue using magnetic bead columns. Isolated microglia can be used to model diseases with neuroinflammatory components for potential therapeutic discoveries. For complete details on the use and execution of this protocol, please refer to Hanamsagar et al. (2017), Rivera et al. (2019), and Edlow et al. (2019).

Full Text

Duke Authors

Cited Authors

  • Bordt, EA; Block, CL; Petrozziello, T; Sadri-Vakili, G; Smith, CJ; Edlow, AG; Bilbo, SD

Published Date

  • June 3, 2020

Published In

Volume / Issue

  • 1 / 1

Start / End Page

  • 100035 -

PubMed ID

  • 32783030

Pubmed Central ID

  • PMC7416840

Electronic International Standard Serial Number (EISSN)

  • 2666-1667

International Standard Serial Number (ISSN)

  • 2666-1667

Digital Object Identifier (DOI)

  • 10.1016/j.xpro.2020.100035

Language

  • eng