Skip to main content

Genome-wide functional analysis of phosphatases in the pathogenic fungus Cryptococcus neoformans.

Publication ,  Journal Article
Jin, J-H; Lee, K-T; Hong, J; Lee, D; Jang, E-H; Kim, J-Y; Lee, Y; Lee, S-H; So, Y-S; Jung, K-W; Lee, D-G; Jeong, E; Lee, M; Jang, Y-B ...
Published in: Nat Commun
August 24, 2020

Phosphatases, together with kinases and transcription factors, are key components in cellular signalling networks. Here, we present a systematic functional analysis of the phosphatases in Cryptococcus neoformans, a fungal pathogen that causes life-threatening fungal meningoencephalitis. We analyse 230 signature-tagged mutant strains for 114 putative phosphatases under 30 distinct in vitro growth conditions, revealing at least one function for 60 of these proteins. Large-scale virulence and infectivity assays using insect and mouse models indicate roles in pathogenicity for 31 phosphatases involved in various processes such as thermotolerance, melanin and capsule production, stress responses, O-mannosylation, or retromer function. Notably, phosphatases Xpp1, Ssu72, Siw14, and Sit4 promote blood-brain barrier adhesion and crossing by C. neoformans. Together with our previous systematic studies of transcription factors and kinases, our results provide comprehensive insight into the pathobiological signalling circuitry of C. neoformans.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Nat Commun

DOI

EISSN

2041-1723

Publication Date

August 24, 2020

Volume

11

Issue

1

Start / End Page

4212

Location

England

Related Subject Headings

  • Virulence
  • Transcription Factors
  • Thermotolerance
  • Signal Transduction
  • Phosphotransferases
  • Phosphoric Monoester Hydrolases
  • Mice, Inbred Strains
  • Genome-Wide Association Study
  • Genome, Fungal
  • Gene Expression Regulation, Fungal
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Jin, J.-H., Lee, K.-T., Hong, J., Lee, D., Jang, E.-H., Kim, J.-Y., … Bahn, Y.-S. (2020). Genome-wide functional analysis of phosphatases in the pathogenic fungus Cryptococcus neoformans. Nat Commun, 11(1), 4212. https://doi.org/10.1038/s41467-020-18028-0
Jin, Jae-Hyung, Kyung-Tae Lee, Joohyeon Hong, Dongpil Lee, Eun-Ha Jang, Jin-Young Kim, Yeonseon Lee, et al. “Genome-wide functional analysis of phosphatases in the pathogenic fungus Cryptococcus neoformans.Nat Commun 11, no. 1 (August 24, 2020): 4212. https://doi.org/10.1038/s41467-020-18028-0.
Jin J-H, Lee K-T, Hong J, Lee D, Jang E-H, Kim J-Y, et al. Genome-wide functional analysis of phosphatases in the pathogenic fungus Cryptococcus neoformans. Nat Commun. 2020 Aug 24;11(1):4212.
Jin, Jae-Hyung, et al. “Genome-wide functional analysis of phosphatases in the pathogenic fungus Cryptococcus neoformans.Nat Commun, vol. 11, no. 1, Aug. 2020, p. 4212. Pubmed, doi:10.1038/s41467-020-18028-0.
Jin J-H, Lee K-T, Hong J, Lee D, Jang E-H, Kim J-Y, Lee Y, Lee S-H, So Y-S, Jung K-W, Lee D-G, Jeong E, Lee M, Jang Y-B, Choi Y, Lee MH, Kim J-S, Yu S-R, Choi J-T, La J-W, Choi H, Kim S-W, Seo KJ, Thak EJ, Choi J, Averette AF, Lee Y-H, Heitman J, Kang HA, Cheong E, Bahn Y-S. Genome-wide functional analysis of phosphatases in the pathogenic fungus Cryptococcus neoformans. Nat Commun. 2020 Aug 24;11(1):4212.

Published In

Nat Commun

DOI

EISSN

2041-1723

Publication Date

August 24, 2020

Volume

11

Issue

1

Start / End Page

4212

Location

England

Related Subject Headings

  • Virulence
  • Transcription Factors
  • Thermotolerance
  • Signal Transduction
  • Phosphotransferases
  • Phosphoric Monoester Hydrolases
  • Mice, Inbred Strains
  • Genome-Wide Association Study
  • Genome, Fungal
  • Gene Expression Regulation, Fungal