A role of the CTCF binding site at enhancer Eα in the dynamic chromatin organization of the Tcra-Tcrd locus.

Journal Article (Journal Article)

The regulation of T cell receptor Tcra gene rearrangement has been extensively studied. The enhancer Eα plays an essential role in Tcra rearrangement by establishing a recombination centre in the Jα array and a chromatin hub for interactions between Vα and Jα genes. But the mechanism of the Eα and its downstream CTCF binding site (here named EACBE) in dynamic chromatin regulation is unknown. The Hi-C data showed that the EACBE is located at the sub-TAD boundary which separates the Tcra-Tcrd locus and the downstream region including the Dad1 gene. The EACBE is required for long-distance regulation of the Eα on the proximal Vα genes, and its deletion impaired the Tcra rearrangement. We also noticed that the EACBE and Eα regulate the genes in the downstream sub-TAD via asymmetric chromatin extrusion. This study provides a new insight into the role of CTCF binding sites at TAD boundaries in gene regulation.

Full Text

Duke Authors

Cited Authors

  • Zhao, H; Li, Z; Zhu, Y; Bian, S; Zhang, Y; Qin, L; Naik, AK; He, J; Zhang, Z; Krangel, MS; Hao, B

Published Date

  • September 25, 2020

Published In

Volume / Issue

  • 48 / 17

Start / End Page

  • 9621 - 9636

PubMed ID

  • 32853367

Pubmed Central ID

  • PMC7515734

Electronic International Standard Serial Number (EISSN)

  • 1362-4962

Digital Object Identifier (DOI)

  • 10.1093/nar/gkaa711


  • eng

Conference Location

  • England