Approaches for Genetic Discoveries in the Skin Commensal and Pathogenic Malassezia Yeasts.

Journal Article (Review;Journal Article)

Malassezia includes yeasts belong to the subphylum Ustilaginomycotina within the Basidiomycota. Malassezia yeasts are commonly found as commensals on human and animal skin. Nevertheless, Malassezia species are also associated with several skin disorders, such as dandruff/seborrheic dermatitis, atopic eczema, pityriasis versicolor, and folliculitis. More recently, associations of Malassezia with Crohn's disease, pancreatic ductal adenocarcinoma, and cystic fibrosis pulmonary exacerbation have been reported. The increasing availability of genomic and molecular tools have played a crucial role in understanding the genetic basis of Malassezia commensalism and pathogenicity. In the present review we report genomics advances in Malassezia highlighting unique features that potentially impacted Malassezia biology and host adaptation. Furthermore, we describe the recently developed protocols for Agrobacterium tumefaciens -mediated transformation in Malassezia , and their applications for random insertional mutagenesis or targeted gene replacement strategies.

Full Text

Duke Authors

Cited Authors

  • Ianiri, G; Heitman, J

Published Date

  • January 2020

Published In

Volume / Issue

  • 10 /

Start / End Page

  • 393 -

PubMed ID

  • 32850491

Pubmed Central ID

  • PMC7426719

Electronic International Standard Serial Number (EISSN)

  • 2235-2988

International Standard Serial Number (ISSN)

  • 2235-2988

Digital Object Identifier (DOI)

  • 10.3389/fcimb.2020.00393

Language

  • eng