Flexible intravenous microdialysis probe for blood sampling in freely moving rats

Journal Article

A flexible intravenous microdialysis probe was constructed from Silastic tubing (0.5 mm ID and 1.0 mm OD), with a cellulose hollow fiber tip 0.2 mm in diameter and 25 mm long with a 6,000 mol wt cut off. In vitro tests showed relative recovery rates of 39.1 +/- 1.9% for epinephrine. In vivo tests in freely moving rats, 36 h and 7 days after surgery, showed stable amounts of epinephrine and glucose. After intraperitoneal injections of 2-deoxy-D-glucose, circulating levels of epinephrine and glucose increased significantly. Similar results were obtained several days after implantation of the probe. We conclude that in situations where prolonged blood sampling is necessary, the flexible microdialysis probe provides a reliable means of accessing circulating levels of neuroactive compounds, nutrients, metabolites, and drugs.

Full Text

Duke Authors

Cited Authors

  • Rada, P; Parada, M; Hernandez, L

Published Date

  • January 1, 1993

Published In

Volume / Issue

  • 74 / 1

Start / End Page

  • 466 - 469

Published By

Electronic International Standard Serial Number (EISSN)

  • 1522-1601

International Standard Serial Number (ISSN)

  • 8750-7587

Digital Object Identifier (DOI)

  • 10.1152/jappl.1993.74.1.466

Language

  • en