Caspase-11-mediated endothelial pyroptosis underlies endotoxemia-induced lung injury.

Journal Article (Journal Article)

Acute lung injury is a leading cause of death in bacterial sepsis due to the wholesale destruction of the lung endothelial barrier, which results in protein-rich lung edema, influx of proinflammatory leukocytes, and intractable hypoxemia. Pyroptosis is a form of programmed lytic cell death that is triggered by inflammatory caspases, but little is known about its role in EC death and acute lung injury. Here, we show that systemic exposure to the bacterial endotoxin lipopolysaccharide (LPS) causes severe endothelial pyroptosis that is mediated by the inflammatory caspases, human caspases 4/5 in human ECs, or the murine homolog caspase-11 in mice in vivo. In caspase-11-deficient mice, BM transplantation with WT hematopoietic cells did not abrogate endotoxemia-induced acute lung injury, indicating a central role for nonhematopoietic caspase-11 in endotoxemia. Additionally, conditional deletion of caspase-11 in ECs reduced endotoxemia-induced lung edema, neutrophil accumulation, and death. These results establish the requisite role of endothelial pyroptosis in endotoxemic tissue injury and suggest that endothelial inflammatory caspases are an important therapeutic target for acute lung injury.

Full Text

Duke Authors

Cited Authors

  • Cheng, KT; Xiong, S; Ye, Z; Hong, Z; Di, A; Tsang, KM; Gao, X; An, S; Mittal, M; Vogel, SM; Miao, EA; Rehman, J; Malik, AB

Published Date

  • November 2017

Published In

Volume / Issue

  • 127 / 11

Start / End Page

  • 4124 - 4135

PubMed ID

  • 28990935

Pubmed Central ID

  • 28990935

Electronic International Standard Serial Number (EISSN)

  • 1558-8238

International Standard Serial Number (ISSN)

  • 0021-9738

Digital Object Identifier (DOI)

  • 10.1172/jci94495

Language

  • eng