Examining metastatic behavior within 3D bioprinted vasculature for the validation of a 3D computational flow model.

Published

Journal Article

Understanding the dynamics of circulating tumor cell (CTC) behavior within the vasculature has remained an elusive goal in cancer biology. To elucidate the contribution of hydrodynamics in determining sites of CTC vascular colonization, the physical forces affecting these cells must be evaluated in a highly controlled manner. To this end, we have bioprinted endothelialized vascular beds and perfused these constructs with metastatic mammary gland cells under physiological flow rates. By pairing these in vitro devices with an advanced computational flow model, we found that the bioprinted analog was readily capable of evaluating the accuracy and integrated complexity of a computational flow model, while also highlighting the discrete contribution of hydrodynamics in vascular colonization. This intersection of these two technologies, bioprinting and computational simulation, is a key demonstration in the establishment of an experimentation pipeline for the understanding of complex biophysical events.

Full Text

Duke Authors

Cited Authors

  • Hynes, WF; Pepona, M; Robertson, C; Alvarado, J; Dubbin, K; Triplett, M; Adorno, JJ; Randles, A; Moya, ML

Published Date

  • August 26, 2020

Published In

Volume / Issue

  • 6 / 35

Start / End Page

  • eabb3308 -

PubMed ID

  • 32923637

Pubmed Central ID

  • 32923637

Electronic International Standard Serial Number (EISSN)

  • 2375-2548

International Standard Serial Number (ISSN)

  • 2375-2548

Digital Object Identifier (DOI)

  • 10.1126/sciadv.abb3308

Language

  • eng