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The interleukin-2 receptor α chain (CD25) plays an important role in regulating monocyte-derived CD40 expression during anti-porcine cellular responses.

Publication ,  Conference
Sun, Z-G; Wang, Z; Zhu, L-M; Fang, Y-S; Yu, L-Z; Xu, H
Published in: Transplant Proc
May 2012

Long-term xenograft survival is limited by delayed xenograft rejection, and monocytes are thought to play an important role in this process. Although typically considered a T cell surface marker, interleukin 2 the receptor chain CD25 is also functional on monocytes. We hypothesized that CD25 expression on monocytes functions to augment monocyte activation in xeno-specific cellular responses. Xenogeneic mixed lymphocyte-endothelial cell reactions were used to study the role of CD25 in facilitating xenogeneic cell-mediated immune responses an in vitro. We also tested the effect of the anti-CD25 antibody daclizumab on monocyte-mediated T cell activation during xeno-specific cellular responses. Co-culture with porcine endothelial cells (PEC) elicited a pronounced proliferative response by human peripheral blood mononuclear cells (PBMC) that was accompanied by upregulation of CD25 and CD40 on CD14(+) monocytes. CD4(+) cells proliferated in response to PEC-conditioned monocytes, while blockade of CD25 with daclizumab reduced CD4(+) cell proliferation in the presence of PEC-conditioned monocytes. In addition, daclizumab inhibited proliferation of PBMC in responses to PEC. Analysis of monocytes from PBMC-PEC cocultures by flow cytometry indicated that daclizumab inhibited CD40 upregulation on PEC-activated monocytes. These data demonstrate that CD25 blockade prevents xenogeneic cellular responses by directly blocking CD25 expression on both activated T cells and monocytes. CD25 blockade on T cells or monocytes may indirectly affect upregulation of CD40 on xenoreactive monocytes. Our data strengthen the rationale for incorporating CD25 directed therapy in discordant xenotransplantation.

Duke Scholars

Published In

Transplant Proc

DOI

EISSN

1873-2623

Publication Date

May 2012

Volume

44

Issue

4

Start / End Page

1139 / 1142

Location

United States

Related Subject Headings

  • Up-Regulation
  • Transplantation, Heterologous
  • Time Factors
  • Swine
  • Monocytes
  • Lymphocyte Activation
  • Lipopolysaccharide Receptors
  • Interleukin-2 Receptor alpha Subunit
  • Interferon-gamma
  • Immunoglobulin G
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Sun, Z.-G., Wang, Z., Zhu, L.-M., Fang, Y.-S., Yu, L.-Z., & Xu, H. (2012). The interleukin-2 receptor α chain (CD25) plays an important role in regulating monocyte-derived CD40 expression during anti-porcine cellular responses. In Transplant Proc (Vol. 44, pp. 1139–1142). United States. https://doi.org/10.1016/j.transproceed.2012.02.014
Sun, Z. -. G., Z. Wang, L. -. M. Zhu, Y. -. S. Fang, L. -. Z. Yu, and H. Xu. “The interleukin-2 receptor α chain (CD25) plays an important role in regulating monocyte-derived CD40 expression during anti-porcine cellular responses.” In Transplant Proc, 44:1139–42, 2012. https://doi.org/10.1016/j.transproceed.2012.02.014.
Sun, Z. .. G., et al. “The interleukin-2 receptor α chain (CD25) plays an important role in regulating monocyte-derived CD40 expression during anti-porcine cellular responses.Transplant Proc, vol. 44, no. 4, 2012, pp. 1139–42. Pubmed, doi:10.1016/j.transproceed.2012.02.014.
Journal cover image

Published In

Transplant Proc

DOI

EISSN

1873-2623

Publication Date

May 2012

Volume

44

Issue

4

Start / End Page

1139 / 1142

Location

United States

Related Subject Headings

  • Up-Regulation
  • Transplantation, Heterologous
  • Time Factors
  • Swine
  • Monocytes
  • Lymphocyte Activation
  • Lipopolysaccharide Receptors
  • Interleukin-2 Receptor alpha Subunit
  • Interferon-gamma
  • Immunoglobulin G