FRET efficiency measurement with a low-cost frequency domain fluorescence lifetime imaging microscope
Publication
, Conference
Dumas, JP; Jiang, JY; Gates, EM; Hoffman, BD; Pierce, MC; Boustany, NN
Published in: Optics InfoBase Conference Papers
January 1, 2020
We utilize a frequency-domain FLIM system operating at a single modulation frequency in combination with a FRET standards calibration curve to measure the FRET efficiency of the vinculin tension sensor, VinTS, at the focal adhesions of living cells.
Duke Scholars
Published In
Optics InfoBase Conference Papers
DOI
EISSN
2162-2701
ISBN
9781943580743
Publication Date
January 1, 2020
Volume
Part F177-Microscopy-2020
Citation
APA
Chicago
ICMJE
MLA
NLM
Dumas, J. P., Jiang, J. Y., Gates, E. M., Hoffman, B. D., Pierce, M. C., & Boustany, N. N. (2020). FRET efficiency measurement with a low-cost frequency domain fluorescence lifetime imaging microscope. In Optics InfoBase Conference Papers (Vol. Part F177-Microscopy-2020). https://doi.org/10.1364/MICROSCOPY.2020.MTu4A.7
Dumas, J. P., J. Y. Jiang, E. M. Gates, B. D. Hoffman, M. C. Pierce, and N. N. Boustany. “FRET efficiency measurement with a low-cost frequency domain fluorescence lifetime imaging microscope.” In Optics InfoBase Conference Papers, Vol. Part F177-Microscopy-2020, 2020. https://doi.org/10.1364/MICROSCOPY.2020.MTu4A.7.
Dumas JP, Jiang JY, Gates EM, Hoffman BD, Pierce MC, Boustany NN. FRET efficiency measurement with a low-cost frequency domain fluorescence lifetime imaging microscope. In: Optics InfoBase Conference Papers. 2020.
Dumas, J. P., et al. “FRET efficiency measurement with a low-cost frequency domain fluorescence lifetime imaging microscope.” Optics InfoBase Conference Papers, vol. Part F177-Microscopy-2020, 2020. Scopus, doi:10.1364/MICROSCOPY.2020.MTu4A.7.
Dumas JP, Jiang JY, Gates EM, Hoffman BD, Pierce MC, Boustany NN. FRET efficiency measurement with a low-cost frequency domain fluorescence lifetime imaging microscope. Optics InfoBase Conference Papers. 2020.
Published In
Optics InfoBase Conference Papers
DOI
EISSN
2162-2701
ISBN
9781943580743
Publication Date
January 1, 2020
Volume
Part F177-Microscopy-2020