FRET efficiency measurement with a low-cost frequency domain fluorescence lifetime imaging microscope

Conference Paper

We utilize a frequency-domain FLIM system operating at a single modulation frequency in combination with a FRET standards calibration curve to measure the FRET efficiency of the vinculin tension sensor, VinTS, at the focal adhesions of living cells.

Full Text

Duke Authors

Cited Authors

  • Dumas, JP; Jiang, JY; Gates, EM; Hoffman, BD; Pierce, MC; Boustany, NN

Published Date

  • January 1, 2020

Published In

  • Optics Infobase Conference Papers

Volume / Issue

  • Part F177-Microscopy-2020 /

International Standard Book Number 13 (ISBN-13)

  • 9781557528209

Digital Object Identifier (DOI)

  • 10.1364/MICROSCOPY.2020.MTu4A.7

Citation Source

  • Scopus