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Cytoplasmic sharing through apical membrane remodeling.

Publication ,  Journal Article
Peterson, NG; Stormo, BM; Schoenfelder, KP; King, JS; Lee, RR; Fox, DT
Published in: Elife
October 14, 2020

Multiple nuclei sharing a common cytoplasm are found in diverse tissues, organisms, and diseases. Yet, multinucleation remains a poorly understood biological property. Cytoplasm sharing invariably involves plasma membrane breaches. In contrast, we discovered cytoplasm sharing without membrane breaching in highly resorptive Drosophila rectal papillae. During a six-hour developmental window, 100 individual papillar cells assemble a multinucleate cytoplasm, allowing passage of proteins of at least 62 kDa throughout papillar tissue. Papillar cytoplasm sharing does not employ canonical mechanisms such as incomplete cytokinesis or muscle fusion pore regulators. Instead, sharing requires gap junction proteins (normally associated with transport of molecules < 1 kDa), which are positioned by membrane remodeling GTPases. Our work reveals a new role for apical membrane remodeling in converting a multicellular epithelium into a giant multinucleate cytoplasm.

Duke Scholars

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Published In

Elife

DOI

EISSN

2050-084X

Publication Date

October 14, 2020

Volume

9

Location

England

Related Subject Headings

  • Signal Transduction
  • Microscopy, Electron, Transmission
  • Intestines
  • Genotype
  • Gap Junctions
  • GTP Phosphohydrolases
  • Epithelium
  • Drosophila melanogaster
  • Cytosol
  • Cytoplasm
 

Citation

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Peterson, N. G., Stormo, B. M., Schoenfelder, K. P., King, J. S., Lee, R. R., & Fox, D. T. (2020). Cytoplasmic sharing through apical membrane remodeling. Elife, 9. https://doi.org/10.7554/eLife.58107
Peterson, Nora G., Benjamin M. Stormo, Kevin P. Schoenfelder, Juliet S. King, Rayson Rs Lee, and Donald T. Fox. “Cytoplasmic sharing through apical membrane remodeling.Elife 9 (October 14, 2020). https://doi.org/10.7554/eLife.58107.
Peterson NG, Stormo BM, Schoenfelder KP, King JS, Lee RR, Fox DT. Cytoplasmic sharing through apical membrane remodeling. Elife. 2020 Oct 14;9.
Peterson, Nora G., et al. “Cytoplasmic sharing through apical membrane remodeling.Elife, vol. 9, Oct. 2020. Pubmed, doi:10.7554/eLife.58107.
Peterson NG, Stormo BM, Schoenfelder KP, King JS, Lee RR, Fox DT. Cytoplasmic sharing through apical membrane remodeling. Elife. 2020 Oct 14;9.

Published In

Elife

DOI

EISSN

2050-084X

Publication Date

October 14, 2020

Volume

9

Location

England

Related Subject Headings

  • Signal Transduction
  • Microscopy, Electron, Transmission
  • Intestines
  • Genotype
  • Gap Junctions
  • GTP Phosphohydrolases
  • Epithelium
  • Drosophila melanogaster
  • Cytosol
  • Cytoplasm