Cytoplasmic sharing through apical membrane remodeling.

Journal Article (Journal Article)

Multiple nuclei sharing a common cytoplasm are found in diverse tissues, organisms, and diseases. Yet, multinucleation remains a poorly understood biological property. Cytoplasm sharing invariably involves plasma membrane breaches. In contrast, we discovered cytoplasm sharing without membrane breaching in highly resorptive Drosophila rectal papillae. During a six-hour developmental window, 100 individual papillar cells assemble a multinucleate cytoplasm, allowing passage of proteins of at least 62 kDa throughout papillar tissue. Papillar cytoplasm sharing does not employ canonical mechanisms such as incomplete cytokinesis or muscle fusion pore regulators. Instead, sharing requires gap junction proteins (normally associated with transport of molecules < 1 kDa), which are positioned by membrane remodeling GTPases. Our work reveals a new role for apical membrane remodeling in converting a multicellular epithelium into a giant multinucleate cytoplasm.

Full Text

Duke Authors

Cited Authors

  • Peterson, NG; Stormo, BM; Schoenfelder, KP; King, JS; Lee, RR; Fox, DT

Published Date

  • October 14, 2020

Published In

Volume / Issue

  • 9 /

PubMed ID

  • 33051002

Pubmed Central ID

  • PMC7655102

Electronic International Standard Serial Number (EISSN)

  • 2050-084X

Digital Object Identifier (DOI)

  • 10.7554/eLife.58107


  • eng

Conference Location

  • England