Phosphoproteomics for the masses.

Journal Article (Journal Article;Review)

Protein phosphorylation serves as a primary mechanism of signal transduction in the cells of biological organisms. Technical advancements over the last several years in mass spectrometry now allow for the large-scale identification and quantitation of in vivo phosphorylation at unprecedented levels. These developments have occurred in the areas of sample preparation, instrumentation, quantitative methodology, and informatics so that today, 10 000-20 000 phosphorylation sites can be identified and quantified within a few weeks. With the rapid development and widespread availability of such data, its translation into biological insight and knowledge is a current obstacle. Here we present an overview of how this technology came to be and is currently applied, as well as future challenges for the field.

Full Text

Duke Authors

Cited Authors

  • Grimsrud, PA; Swaney, DL; Wenger, CD; Beauchene, NA; Coon, JJ

Published Date

  • January 15, 2010

Published In

Volume / Issue

  • 5 / 1

Start / End Page

  • 105 - 119

PubMed ID

  • 20047291

Pubmed Central ID

  • PMC2810156

Electronic International Standard Serial Number (EISSN)

  • 1554-8937

Digital Object Identifier (DOI)

  • 10.1021/cb900277e


  • eng

Conference Location

  • United States