Designing topographically textured microparticles for induction and modulation of osteogenesis in mesenchymal stem cell engineering.

Journal Article (Journal Article)

Mesenchymal stem cells are the focus of intense research in bone development and regeneration. The potential of microparticles as modulating moieties of osteogenic response by utilizing their architectural features is demonstrated herein. Topographically textured microparticles of varying microscale features are produced by exploiting phase-separation of a readily soluble sacrificial component from polylactic acid. The influence of varying topographical features on primary human mesenchymal stem cell attachment, proliferation and markers of osteogenesis is investigated. In the absence of osteoinductive supplements, cells cultured on textured microparticles exhibit notably increased expression of osteogenic markers relative to conventional smooth microparticles. They also exhibit varying morphological, attachment and proliferation responses. Significantly altered gene expression and metabolic profiles are observed, with varying histological characteristics in vivo. This study highlights how tailoring topographical design offers cell-instructive 3D microenvironments which allow manipulation of stem cell fate by eliciting the desired downstream response without use of exogenous osteoinductive factors.

Full Text

Duke Authors

Cited Authors

  • Amer, MH; Alvarez-Paino, M; McLaren, J; Pappalardo, F; Trujillo, S; Wong, JQ; Shrestha, S; Abdelrazig, S; Stevens, LA; Lee, JB; Kim, D-H; González-García, C; Needham, D; Salmerón-Sánchez, M; Shakesheff, KM; Alexander, MR; Alexander, C; Rose, FR

Published Date

  • January 2021

Published In

Volume / Issue

  • 266 /

Start / End Page

  • 120450 -

PubMed ID

  • 33096376

Electronic International Standard Serial Number (EISSN)

  • 1878-5905

International Standard Serial Number (ISSN)

  • 0142-9612

Digital Object Identifier (DOI)

  • 10.1016/j.biomaterials.2020.120450

Language

  • eng