Protocol to Generate Senescent Cells from the Mouse Hepatic Cell Line AML12 to Study Hepatic Aging.

Journal Article (Journal Article)

Previously developed senescent primary fibroblast models have limited relevance to study age-related changes in metabolically active tissues such as the liver. Here, we describe a protocol to generate senescent cells from the mouse hepatic cell line, AML12. These senescent cells exhibit molecular and metabolic signatures that are similar to those observed in livers from aged mice. These senescent AML12 cells should be a useful in vitro model to study the metabolic effects of aging in the liver. For complete details on the use and execution of this protocol, please refer to Singh et al. (2020).

Full Text

Duke Authors

Cited Authors

  • Tripathi, M; Yen, PM; Singh, BK

Published Date

  • September 18, 2020

Published In

Volume / Issue

  • 1 / 2

Start / End Page

  • 100064 -

PubMed ID

  • 33111102

Pubmed Central ID

  • PMC7580094

Electronic International Standard Serial Number (EISSN)

  • 2666-1667

Digital Object Identifier (DOI)

  • 10.1016/j.xpro.2020.100064


  • eng

Conference Location

  • United States