Deterministic droplet coding via acoustofluidics.

Journal Article (Journal Article)

Droplet microfluidics has become an indispensable tool for biomedical research and lab-on-a-chip applications owing to its unprecedented throughput, precision, and cost-effectiveness. Although droplets can be generated and screened in a high-throughput manner, the inability to label the inordinate amounts of droplets hinders identifying the individual droplets after generation. Herein, we demonstrate an acoustofluidic platform that enables on-demand, real-time dispensing, and deterministic coding of droplets based on their volumes. By dynamically splitting the aqueous flow using an oil jet triggered by focused traveling surface acoustic waves, a sequence of droplets with deterministic volumes can be continuously dispensed at a throughput of 100 Hz. These sequences encode barcoding information through the combination of various droplet lengths. As a proof-of-concept, we encoded droplet sequences into end-to-end packages (e.g., a series of 50 droplets), which consisted of an address barcode with binary volumetric combinations and a sample package with consistent volumes for hosting analytes. This acoustofluidics-based, deterministic droplet coding technique enables the tagging of droplets with high capacity and high error-tolerance, and can potentially benefit various applications involving single cell phenotyping and multiplexed screening.

Full Text

Duke Authors

Cited Authors

  • Zhang, P; Wang, W; Fu, H; Rich, J; Su, X; Bachman, H; Xia, J; Zhang, J; Zhao, S; Zhou, J; Huang, TJ

Published Date

  • November 2020

Published In

Volume / Issue

  • 20 / 23

Start / End Page

  • 4466 - 4473

PubMed ID

  • 33103674

Pubmed Central ID

  • PMC7688411

Electronic International Standard Serial Number (EISSN)

  • 1473-0189

International Standard Serial Number (ISSN)

  • 1473-0197

Digital Object Identifier (DOI)

  • 10.1039/d0lc00538j

Language

  • eng