Protocol for Recording from Ventral Tegmental Area Dopamine Neurons in Mice while Measuring Force during Head-Fixation.

Journal Article (Journal Article)

Many studies in systems neuroscience use head-fixation preparations for in vivo experimentation. While head-fixation confers several advantages, one major limitation is the lack of behavioral measures that quantify whole-body movements. Here, we detail a step-by-step protocol for using a novel head-fixation device that measures the forces exerted by head-fixed mice in multiple dimensions. We further detail how this system can be used in conjunction with in vivo electrophysiology and optogenetics to study dopamine neurons in the ventral tegmental area. For complete details on the use and execution of this protocol, please refer to Hughes et al. (2020a, 2020b).

Full Text

Duke Authors

Cited Authors

  • Bakhurin, KI; Hughes, RN; Barter, JW; Zhang, J; Yin, HH

Published Date

  • September 2020

Published In

Volume / Issue

  • 1 / 2

Start / End Page

  • 100091 -

PubMed ID

  • 33111123

Pubmed Central ID

  • PMC7580214

Electronic International Standard Serial Number (EISSN)

  • 2666-1667

International Standard Serial Number (ISSN)

  • 2666-1667

Digital Object Identifier (DOI)

  • 10.1016/j.xpro.2020.100091


  • eng