An Aptamer for Broad Cancer Targeting and Therapy.

Journal Article (Journal Article)

Recent advances in chemotherapy treatments are increasingly targeted therapies, with the drug conjugated to an antibody able to deliver it directly to the tumor. As high-affinity chemical ligands that are much smaller in size, aptamers are ideal for this type of drug targeting. Aptamer-highly toxic drug conjugates (ApTDCs) based on the E3 aptamer, selected on prostate cancer cells, target and inhibit prostate tumor growth in vivo. Here, we observe that E3 also broadly targets numerous other cancer types, apparently representing a universal aptamer for cancer targeting. Accordingly, ApTDCs formed by conjugation of E3 to the drugs monomethyl auristatin E (MMAE) or monomethyl auristatin F (MMAF) efficiently target and kill a range of different cancer cells. Notably, this targeting extends to both patient-derived explant (PDX) cancer cell lines and tumors, with the E3 MMAE and MMAF conjugates inhibiting PDX cell growth in vitro and with the E3 aptamer targeting PDX colorectal tumors in vivo.

Full Text

Duke Authors

Cited Authors

  • Powell Gray, B; Song, X; Hsu, DS; Kratschmer, C; Levy, M; Barry, AP; Sullenger, BA

Published Date

  • October 31, 2020

Published In

Volume / Issue

  • 12 / 11

PubMed ID

  • 33142831

Pubmed Central ID

  • PMC7694147

International Standard Serial Number (ISSN)

  • 2072-6694

Digital Object Identifier (DOI)

  • 10.3390/cancers12113217

Language

  • eng

Conference Location

  • Switzerland