Cell wall remodeling and vesicle trafficking mediate the root clock in Arabidopsis .

Journal Article (Journal Article)

In Arabidopsis thaliana , lateral roots initiate in a process preceded by periodic gene expression known as the root clock. We identified the vesicle-trafficking regulator GNOM and its suppressor, ADENOSINE PHOSPHATE RIBOSYLATION FACTOR GTPase ACTIVATION PROTEIN DOMAIN3, as root clock regulators. GNOM is required for the proper distribution of pectin, a mediator of intercellular adhesion, whereas the pectin esterification state is essential for a functional root clock. In sites of lateral root primordia emergence, both esterified and de-esterified pectin variants are differentially distributed. Using a reverse-genetics approach, we show that genes controlling pectin esterification regulate the root clock and lateral root initiation. These results indicate that the balance between esterified and de-esterified pectin states is essential for proper root clock function and the subsequent initiation of lateral root primordia.

Full Text

Duke Authors

Cited Authors

  • Wachsman, G; Zhang, J; Moreno-Risueno, MA; Anderson, CT; Benfey, PN

Published Date

  • November 2020

Published In

Volume / Issue

  • 370 / 6518

Start / End Page

  • 819 - 823

PubMed ID

  • 33184208

Pubmed Central ID

  • PMC7758939

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.abb7250

Language

  • eng