Cell wall remodeling and vesicle trafficking mediate the root clock in Arabidopsis .
Journal Article (Journal Article)
In Arabidopsis thaliana , lateral roots initiate in a process preceded by periodic gene expression known as the root clock. We identified the vesicle-trafficking regulator GNOM and its suppressor, ADENOSINE PHOSPHATE RIBOSYLATION FACTOR GTPase ACTIVATION PROTEIN DOMAIN3, as root clock regulators. GNOM is required for the proper distribution of pectin, a mediator of intercellular adhesion, whereas the pectin esterification state is essential for a functional root clock. In sites of lateral root primordia emergence, both esterified and de-esterified pectin variants are differentially distributed. Using a reverse-genetics approach, we show that genes controlling pectin esterification regulate the root clock and lateral root initiation. These results indicate that the balance between esterified and de-esterified pectin states is essential for proper root clock function and the subsequent initiation of lateral root primordia.
Full Text
Duke Authors
Cited Authors
- Wachsman, G; Zhang, J; Moreno-Risueno, MA; Anderson, CT; Benfey, PN
Published Date
- November 2020
Published In
Volume / Issue
- 370 / 6518
Start / End Page
- 819 - 823
PubMed ID
- 33184208
Pubmed Central ID
- PMC7758939
Electronic International Standard Serial Number (EISSN)
- 1095-9203
International Standard Serial Number (ISSN)
- 0036-8075
Digital Object Identifier (DOI)
- 10.1126/science.abb7250
Language
- eng