Demineralized Murine Skeletal Histology.

Journal Article (Journal Article)

Cartilage and bone are specialized skeletal tissues composed of unique extracellular matrices. Bone, in particular, has a highly calcified or mineralized matrix that makes microtomy and standard histological studies very challenging. Therefore, methods to appropriately fix and decalcify mineralized skeletal tissues have been developed to allow for paraffin processing and standard microtomy. In this chapter, we will illustrate methods for tissue grossing, fixation, decalcification, paraffin processing, embedding, sectioning, and routine histological staining of demineralized murine skeletal tissues. We will also discuss methods for decalcified frozen sectioning of skeletal tissues with and without the use of a tape-transfer system.

Full Text

Duke Authors

Cited Authors

  • Mirando, AJ; Hilton, MJ

Published Date

  • 2021

Published In

Volume / Issue

  • 2230 /

Start / End Page

  • 283 - 302

PubMed ID

  • 33197020

Pubmed Central ID

  • 33197020

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Digital Object Identifier (DOI)

  • 10.1007/978-1-0716-1028-2_16

Language

  • eng

Conference Location

  • United States