Whole Mount In Situ Hybridization in Murine Tissues.

Journal Article (Journal Article)

Whole mount in situ hybridization is a sensitive method used to characterize the spatial and temporal expression of RNA transcripts throughout an entire tissue. This method is an excellent tool for studying gene expression during embryonic development. Here, we describe a procedure for digoxigenin labeled in situ hybridization on whole embryos.

Full Text

Duke Authors

Cited Authors

  • Sharma, D; Hilton, MJ; Karner, CM

Published Date

  • 2021

Published In

Volume / Issue

  • 2230 /

Start / End Page

  • 367 - 376

PubMed ID

  • 33197026

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Digital Object Identifier (DOI)

  • 10.1007/978-1-0716-1028-2_22

Language

  • eng

Conference Location

  • United States