Isolation and Culture of Murine Primary Chondrocytes: Costal and Growth Plate Cartilage.

Journal Article (Journal Article)

Primary chondrocyte isolation and culture is a useful tool to characterize how cellular perturbations impact chondrocyte behavior and mineralization in vitro. This protocol conveys methods for isolating and culturing primary chondrocytes from costal and growth plate cartilage. Following gross dissection of the neonatal murine anterior rib cage or long bone growth plate cartilage, chondrocytes are isolated via enzymatic digestion and plated at high density. Genetic perturbation of plated primary murine chondrocytes using viral infection of Cre recombinase to excise floxed alleles and/or overexpress genes of interest are also described.

Full Text

Duke Authors

Cited Authors

  • Liao, Y; Long, JT; Gallo, CJR; Mirando, AJ; Hilton, MJ

Published Date

  • 2021

Published In

Volume / Issue

  • 2230 /

Start / End Page

  • 415 - 423

PubMed ID

  • 33197029

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Digital Object Identifier (DOI)

  • 10.1007/978-1-0716-1028-2_25

Language

  • eng

Conference Location

  • United States