Isolation and Culture of Murine Primary Chondrocytes: Costal and Growth Plate Cartilage.
Journal Article (Journal Article)
Primary chondrocyte isolation and culture is a useful tool to characterize how cellular perturbations impact chondrocyte behavior and mineralization in vitro. This protocol conveys methods for isolating and culturing primary chondrocytes from costal and growth plate cartilage. Following gross dissection of the neonatal murine anterior rib cage or long bone growth plate cartilage, chondrocytes are isolated via enzymatic digestion and plated at high density. Genetic perturbation of plated primary murine chondrocytes using viral infection of Cre recombinase to excise floxed alleles and/or overexpress genes of interest are also described.
Full Text
Duke Authors
Cited Authors
- Liao, Y; Long, JT; Gallo, CJR; Mirando, AJ; Hilton, MJ
Published Date
- 2021
Published In
Volume / Issue
- 2230 /
Start / End Page
- 415 - 423
PubMed ID
- 33197029
Electronic International Standard Serial Number (EISSN)
- 1940-6029
Digital Object Identifier (DOI)
- 10.1007/978-1-0716-1028-2_25
Language
- eng
Conference Location
- United States