Prepronociceptin-Expressing Neurons in the Extended Amygdala Encode and Promote Rapid Arousal Responses to Motivationally Salient Stimuli.

Journal Article (Journal Article)

Motivational states consist of cognitive, emotional, and physiological components controlled by multiple brain regions. An integral component of this neural circuitry is the bed nucleus of the stria terminalis (BNST). Here, we identify that neurons within BNST that express the gene prepronociceptin (PnocBNST ) modulate rapid changes in physiological arousal that occur upon exposure to motivationally salient stimuli. Using in vivo two-photon calcium imaging, we find that PnocBNST neuronal responses directly correspond with rapid increases in pupillary size when mice are exposed to aversive and rewarding odors. Furthermore, optogenetic activation of these neurons increases pupillary size and anxiety-like behaviors but does not induce approach, avoidance, or locomotion. These findings suggest that excitatory responses in PnocBNST neurons encode rapid arousal responses that modulate anxiety states. Further histological, electrophysiological, and single-cell RNA sequencing data reveal that PnocBNST neurons are composed of genetically and anatomically identifiable subpopulations that may differentially tune rapid arousal responses to motivational stimuli.

Full Text

Duke Authors

Cited Authors

  • Rodriguez-Romaguera, J; Ung, RL; Nomura, H; Otis, JM; Basiri, ML; Namboodiri, VMK; Zhu, X; Robinson, JE; van den Munkhof, HE; McHenry, JA; Eckman, LEH; Kosyk, O; Jhou, TC; Kash, TL; Bruchas, MR; Stuber, GD

Published Date

  • November 2020

Published In

Volume / Issue

  • 33 / 6

Start / End Page

  • 108362 -

PubMed ID

  • 33176134

Pubmed Central ID

  • PMC8136285

Electronic International Standard Serial Number (EISSN)

  • 2211-1247

International Standard Serial Number (ISSN)

  • 2211-1247

Digital Object Identifier (DOI)

  • 10.1016/j.celrep.2020.108362

Language

  • eng