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An important role of a pyrethroid-sensing residue F1519 in the action of the N-alkylamide insecticide BTG 502 on the cockroach sodium channel.

Publication ,  Journal Article
Du, Y; Khambay, B; Dong, K
Published in: Insect biochemistry and molecular biology
July 2011

Deltamethrin, a pyrethroid insecticide, and BTG 502, an alkylamide insecticide, target voltage-gated sodium channels. Deltamethrin binds to a unique receptor site and causes prolonged opening of sodium channels by inhibiting deactivation and inactivation. Previous (22)Na(+) influx and receptor binding assays using mouse brain synaptoneurosomes showed that BTG 502 antagonized the binding and action of batrachotoxin (BTX), a site 2 sodium channel neurotoxin. However, the effect of BTG 502 has not been examined directly on sodium channels expressed in Xenopus oocytes. In this study, we examined the effect of BTG 502 on wild-type and mutant cockroach sodium channels expressed in Xenopus oocytes. Toxin competition experiments confirmed that BTG 502 antagonizes the action of BTX and possibly shares a common receptor site with BTX. However, unlike BTX which causes persistent activation of sodium channels, BTG 502 reduces the amplitude of peak sodium current. A previous study showed that BTG 502 was more toxic to pyrethroid-resistant house flies possessing a super-kdr (knockdown resistance) mechanism than to pyrethroid-susceptible house flies. However, we found that the cockroach sodium channels carrying the equivalent super-kdr mutations (M918T and L1014F) were not more sensitive to BTG 502 than the wild-type channel. Instead, a kdr mutation, F1519I, which reduces pyrethroid binding, abolished the action of BTG 502. These results provide evidence the actions of alkylamide and pyrethroid insecticides require a common sodium channel residue.

Duke Scholars

Published In

Insect biochemistry and molecular biology

DOI

EISSN

1879-0240

ISSN

0965-1748

Publication Date

July 2011

Volume

41

Issue

7

Start / End Page

446 / 450

Related Subject Headings

  • Xenopus laevis
  • Transfection
  • Sodium Channels
  • Sodium Channel Agonists
  • Sodium
  • Recombinant Proteins
  • Rats
  • Pyrethrins
  • Protein Binding
  • Polyunsaturated Alkamides
 

Citation

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Du, Y., Khambay, B., & Dong, K. (2011). An important role of a pyrethroid-sensing residue F1519 in the action of the N-alkylamide insecticide BTG 502 on the cockroach sodium channel. Insect Biochemistry and Molecular Biology, 41(7), 446–450. https://doi.org/10.1016/j.ibmb.2011.03.005
Du, Yuzhe, Bhupinder Khambay, and Ke Dong. “An important role of a pyrethroid-sensing residue F1519 in the action of the N-alkylamide insecticide BTG 502 on the cockroach sodium channel.Insect Biochemistry and Molecular Biology 41, no. 7 (July 2011): 446–50. https://doi.org/10.1016/j.ibmb.2011.03.005.
Du, Yuzhe, et al. “An important role of a pyrethroid-sensing residue F1519 in the action of the N-alkylamide insecticide BTG 502 on the cockroach sodium channel.Insect Biochemistry and Molecular Biology, vol. 41, no. 7, July 2011, pp. 446–50. Epmc, doi:10.1016/j.ibmb.2011.03.005.
Journal cover image

Published In

Insect biochemistry and molecular biology

DOI

EISSN

1879-0240

ISSN

0965-1748

Publication Date

July 2011

Volume

41

Issue

7

Start / End Page

446 / 450

Related Subject Headings

  • Xenopus laevis
  • Transfection
  • Sodium Channels
  • Sodium Channel Agonists
  • Sodium
  • Recombinant Proteins
  • Rats
  • Pyrethrins
  • Protein Binding
  • Polyunsaturated Alkamides