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Persistent tetrodotoxin-sensitive sodium current resulting from U-to-C RNA editing of an insect sodium channel.

Publication ,  Journal Article
Liu, Z; Song, W; Dong, K
Published in: Proceedings of the National Academy of Sciences of the United States of America
August 2004

The persistent tetrodotoxin (TTX)-sensitive sodium current, detected in neurons of many regions of mammalian brains, is associated with many essential neuronal activities, including boosting of excitatory synaptic inputs, acceleration of firing rates, and promotion of oscillatory neuronal activities. However, the origin and molecular basis of the persistent current have remained controversial for decades. Here, we provide direct evidence that U-to-C RNA editing of an insect sodium channel transcript generates a sodium channel with a persistent current. We detected a persistent TTX-sensitive current in a splice variant of the cockroach sodium channel gene BgNa(v) (formerly para(CSMA)). Site-directed mutagenesis experiments revealed that an F-to-S change at the C-terminal domain of this variant was responsible for the persistent current. We demonstrated that this F-to-S change was the result of a U-to-C RNA editing event, which also occurred in the Drosophila para sodium channel transcript. Our work provides direct support for the hypothesis that posttranscriptional modification of a conventional transient sodium channel produces a persistent TTX-sensitive sodium channel.

Duke Scholars

Published In

Proceedings of the National Academy of Sciences of the United States of America

DOI

EISSN

1091-6490

ISSN

0027-8424

Publication Date

August 2004

Volume

101

Issue

32

Start / End Page

11862 / 11867

Related Subject Headings

  • Tetrodotoxin
  • Sodium Channels
  • Sodium
  • RNA Editing
  • Protein Isoforms
  • Neuropeptides
  • Mutagenesis, Site-Directed
  • Insecta
  • Genetic Variation
  • Cockroaches
 

Citation

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MLA
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Liu, Z., Song, W., & Dong, K. (2004). Persistent tetrodotoxin-sensitive sodium current resulting from U-to-C RNA editing of an insect sodium channel. Proceedings of the National Academy of Sciences of the United States of America, 101(32), 11862–11867. https://doi.org/10.1073/pnas.0307695101
Liu, Zhiqi, Weizhong Song, and Ke Dong. “Persistent tetrodotoxin-sensitive sodium current resulting from U-to-C RNA editing of an insect sodium channel.Proceedings of the National Academy of Sciences of the United States of America 101, no. 32 (August 2004): 11862–67. https://doi.org/10.1073/pnas.0307695101.
Liu Z, Song W, Dong K. Persistent tetrodotoxin-sensitive sodium current resulting from U-to-C RNA editing of an insect sodium channel. Proceedings of the National Academy of Sciences of the United States of America. 2004 Aug;101(32):11862–7.
Liu, Zhiqi, et al. “Persistent tetrodotoxin-sensitive sodium current resulting from U-to-C RNA editing of an insect sodium channel.Proceedings of the National Academy of Sciences of the United States of America, vol. 101, no. 32, Aug. 2004, pp. 11862–67. Epmc, doi:10.1073/pnas.0307695101.
Liu Z, Song W, Dong K. Persistent tetrodotoxin-sensitive sodium current resulting from U-to-C RNA editing of an insect sodium channel. Proceedings of the National Academy of Sciences of the United States of America. 2004 Aug;101(32):11862–11867.
Journal cover image

Published In

Proceedings of the National Academy of Sciences of the United States of America

DOI

EISSN

1091-6490

ISSN

0027-8424

Publication Date

August 2004

Volume

101

Issue

32

Start / End Page

11862 / 11867

Related Subject Headings

  • Tetrodotoxin
  • Sodium Channels
  • Sodium
  • RNA Editing
  • Protein Isoforms
  • Neuropeptides
  • Mutagenesis, Site-Directed
  • Insecta
  • Genetic Variation
  • Cockroaches