Novel point mutations in the German cockroach para sodium channel gene are associated with knockdown resistance (kdr) to pyrethroid insecticides.

Journal Article (Journal Article)

Knockdown resistance (kdr) to pyrethroid insecticides has been attributed to point mutations in the para sodium channel gene in more than a half dozen insect pest species. In this study, we identified two novel para mutations in five highly resistant kdr-type German cockroach strains. The two mutations, from glutamic acid (E434) to lysine (K434) and from cysteine (C764) to arginine (R764), respectively, are located in the first intracellular linker connecting domains I and II. E434K is located near the beginning of the linker (closest to domain I), whereas C764R is found toward the end of the linker (closest to domain II). Two additional mutations from aspartic acid (D58) to glycine (G58), and from proline (P1880) to leucine (L1888), respectively, were found in one of the resistant strains. The four mutations coexist with the previously identified leucine to phenylalanine (L993F) kdr mutation in IIS6, and are present only in the highly resistant individuals of a given strain. These findings suggest that these mutations might be responsible for high levels of knockdown resistance toward pyrethroid insecticides in the German cockroach.

Full Text

Duke Authors

Cited Authors

  • Liu, Z; Valles, SM; Dong, K

Published Date

  • October 2000

Published In

Volume / Issue

  • 30 / 10

Start / End Page

  • 991 - 997

PubMed ID

  • 10899465

Pubmed Central ID

  • PMC3049294

Electronic International Standard Serial Number (EISSN)

  • 1879-0240

International Standard Serial Number (ISSN)

  • 0965-1748

Digital Object Identifier (DOI)

  • 10.1016/s0965-1748(00)00074-6


  • eng