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Mechanisms responsible for cypermethrin resistance in a strain of German cockroach, Blattella germanica

Publication ,  Journal Article
Valles, SM; Dong, K; Brenner, RJ
Published in: Pesticide Biochemistry and Physiology
January 1, 2000

Cypermethrin resistance level and mechanisms responsible for the resistance were investigated in a strain (Aves) of German cockroach, Blattella germanica (L.), collected recently from a Gainesville, Florida residence. Topical bioassay data revealed that the Aves strain was highly resistant to cypermethrin, exhibiting a resistance ratio of 93-fold, which was reduced to 29-fold when cockroaches were pretreated with piperonyl butoxide and reduced to 18-fold when pretreated with S,S,S-tributyl phosphorotrithioate. The synergist data implicated enhanced oxidative and hydrolytic metabolism as resistance mechanisms in the Aves German cockroach strain. This conclusion was further supported by significantly higher oxidative (2.4- to 4.2-fold) and hydrolytic (1.6- to 3.6-fold) detoxification enzyme activities toward surrogate substrates and significantly higher in vitro [14C]cypermethrin metabolism. Microsomal NADPH-dependent (1.8-fold) and NADPH-independent (2.2-fold) [14C]cypermethrin metabolism were significantly greater in the Aves strain than in the Orlando insecticide-susceptible strain. In vivo penetration studies with [14C]cypermethrin indicated that decreased cypermethrin penetration may also be a contributing resistance mechanism in the Aves strain. Finally, the Leu993Phe mutation, shown previously to be associated with knockdown resistance (kdr), was present in the Aves strain. (C) 2000 Academic Press.

Duke Scholars

Published In

Pesticide Biochemistry and Physiology

DOI

ISSN

0048-3575

Publication Date

January 1, 2000

Volume

66

Issue

3

Start / End Page

195 / 205

Related Subject Headings

  • Entomology
  • 07 Agricultural and Veterinary Sciences
  • 06 Biological Sciences
 

Citation

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Valles, S. M., Dong, K., & Brenner, R. J. (2000). Mechanisms responsible for cypermethrin resistance in a strain of German cockroach, Blattella germanica. Pesticide Biochemistry and Physiology, 66(3), 195–205. https://doi.org/10.1006/pest.1999.2462
Valles, S. M., K. Dong, and R. J. Brenner. “Mechanisms responsible for cypermethrin resistance in a strain of German cockroach, Blattella germanica.” Pesticide Biochemistry and Physiology 66, no. 3 (January 1, 2000): 195–205. https://doi.org/10.1006/pest.1999.2462.
Valles SM, Dong K, Brenner RJ. Mechanisms responsible for cypermethrin resistance in a strain of German cockroach, Blattella germanica. Pesticide Biochemistry and Physiology. 2000 Jan 1;66(3):195–205.
Valles, S. M., et al. “Mechanisms responsible for cypermethrin resistance in a strain of German cockroach, Blattella germanica.” Pesticide Biochemistry and Physiology, vol. 66, no. 3, Jan. 2000, pp. 195–205. Scopus, doi:10.1006/pest.1999.2462.
Valles SM, Dong K, Brenner RJ. Mechanisms responsible for cypermethrin resistance in a strain of German cockroach, Blattella germanica. Pesticide Biochemistry and Physiology. 2000 Jan 1;66(3):195–205.
Journal cover image

Published In

Pesticide Biochemistry and Physiology

DOI

ISSN

0048-3575

Publication Date

January 1, 2000

Volume

66

Issue

3

Start / End Page

195 / 205

Related Subject Headings

  • Entomology
  • 07 Agricultural and Veterinary Sciences
  • 06 Biological Sciences