Reduced plasmablast frequency is associated with seronegative myasthenia gravis.

Journal Article (Journal Article)

BACKGROUND: The immunopathology of autoimmune seronegative myasthenia gravis (SN MG) is poorly understood. Our objective was to determine immune profiles associated with a diagnosis of SN MG. METHODS: We performed high-dimensional flow cytometry on blood samples from SN MG patients (N = 68), healthy controls (N = 46), and acetylcholine receptor antibody (AChR+) MG patients (N = 27). We compared 12 immune cell subsets in SN MG to controls using logistic modeling via a discovery-replication design. An exploratory analysis fit a multinomial model comparing AChR+ MG and controls to SN MG. RESULTS: An increase in CD19+ CD20- CD38hi plasmablast frequencies was associated with lower odds of being a SN MG case in both the discovery and replication analyses (discovery P-value = .0003, replication P-value = .0021). Interleukin (IL) -21 producing helper T cell frequencies were associated with a diagnosis of AChR+ MG (P = .004). CONCLUSIONS: Reduced plasmablast frequencies are strongly associated with a SN MG diagnosis and may be a useful diagnostic biomarker in the future.

Full Text

Duke Authors

Cited Authors

  • Guptill, JT; Barfield, R; Chan, C; Russo, MA; Emmett, D; Raja, S; Massey, JM; Juel, VC; Hobson-Webb, LD; Gable, KL; Gonzalez, N; Hammett, A; Howard, JF; Chopra, M; Kaminski, HJ; Siddiqi, ZA; Migdal, M; Yi, JS

Published Date

  • April 2021

Published In

Volume / Issue

  • 63 / 4

Start / End Page

  • 577 - 585

PubMed ID

  • 33294984

Pubmed Central ID

  • PMC8100987

Electronic International Standard Serial Number (EISSN)

  • 1097-4598

Digital Object Identifier (DOI)

  • 10.1002/mus.27140


  • eng

Conference Location

  • United States