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Electron microscopy in renal pathology: overall applications and guidelines for tissue, collection, preparation, and stains.

Publication ,  Journal Article
Howell, DN; Herrera, GA
Published in: Ultrastruct Pathol
January 2, 2021

Electron microscopy is a mainstay in the analysis of renal biopsies, where it is typically employed in a correlative fashion along with light and immunofluorescence microscopy. Despite the development of a growing armamentarium of molecular and biochemical analytic methods as well as new immunostains with a widening panel of immunoreactants, electron microscopy remains crucial to the diagnosis of a number of disorders involving the renal glomerulus, vasculature, and tubulointerstitial compartment. The number of renal biopsies continues to grow and the indications for these biopsies continue expanding together with our understanding of disease processes. Proper collection of biopsies and careful analysis of data emanating from diagnostic modalities, clinical information, imaging, gross and microscopic tissue analysis, including a wide range of ancillary studies, represent the essential paradigm for generating detailed diagnoses with clinical significance. This communication offers a guide to the pre-analytic and analytic process for renal biopsy examination, discusses diagnostic keys and pitfalls for an important category of renal diseases (immune complex disorders), and provides an introduction to a useful adjunct diagnostic method (ultrastructural immunolabeling). Renal pathologists should render expert diagnoses that guide patient management, provide prognostic information and lead to targeted new therapeutic interventions that are currently available.

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Published In

Ultrastruct Pathol

DOI

EISSN

1521-0758

Publication Date

January 2, 2021

Volume

45

Issue

1

Start / End Page

1 / 18

Location

England

Related Subject Headings

  • Pathology
  • Microscopy, Electron
  • Kidney Glomerulus
  • Kidney Diseases
  • Kidney
  • Humans
  • Coloring Agents
  • Biopsy
  • 3202 Clinical sciences
  • 1103 Clinical Sciences
 

Citation

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Chicago
ICMJE
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Howell, D. N., & Herrera, G. A. (2021). Electron microscopy in renal pathology: overall applications and guidelines for tissue, collection, preparation, and stains. Ultrastruct Pathol, 45(1), 1–18. https://doi.org/10.1080/01913123.2020.1854407
Howell, David N., and Guillermo A. Herrera. “Electron microscopy in renal pathology: overall applications and guidelines for tissue, collection, preparation, and stains.Ultrastruct Pathol 45, no. 1 (January 2, 2021): 1–18. https://doi.org/10.1080/01913123.2020.1854407.
Howell, David N., and Guillermo A. Herrera. “Electron microscopy in renal pathology: overall applications and guidelines for tissue, collection, preparation, and stains.Ultrastruct Pathol, vol. 45, no. 1, Jan. 2021, pp. 1–18. Pubmed, doi:10.1080/01913123.2020.1854407.

Published In

Ultrastruct Pathol

DOI

EISSN

1521-0758

Publication Date

January 2, 2021

Volume

45

Issue

1

Start / End Page

1 / 18

Location

England

Related Subject Headings

  • Pathology
  • Microscopy, Electron
  • Kidney Glomerulus
  • Kidney Diseases
  • Kidney
  • Humans
  • Coloring Agents
  • Biopsy
  • 3202 Clinical sciences
  • 1103 Clinical Sciences