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High-throughput intracellular biopsy of microRNAs for dissecting the temporal dynamics of cellular heterogeneity.

Publication ,  Journal Article
Wang, Z; Qi, L; Yang, Y; Lu, M; Xie, K; Zhao, X; Cheung, EHC; Wang, Y; Jiang, X; Zhang, W; Huang, L; Wang, X; Shi, P
Published in: Science advances
June 2020

The capability to analyze small RNAs responsible for post-transcriptional regulation of genes expression is essential for characterizing cellular phenotypes. Here, we describe an intracellular biopsy technique (inCell-Biopsy) for fast, multiplexed, and highly sensitive profiling of microRNAs (miRNAs). The technique uses an array of diamond nanoneedles that are functionalized with size-dependent RNA binding proteins, working as "fishing rods" to directly pull miRNAs out of cytoplasm while keeping the cells alive, thus enabling quasi-single-cell miRNA analysis. Each nanoneedle works as a reaction chamber for parallel in situ amplification, visualization, and quantification of miRNAs as low as femtomolar, which is sufficient to detect miRNAs of a single-copy intracellular abundance with specificity to single-nucleotide variation. Using inCell-Biopsy, we analyze the temporal miRNA transcriptome over the differentiation of embryonic stem cells (ESCs). The combinatorial miRNA expression patterns derived by inCell-Biopsy identify emerging cell subpopulations differentiated from ESCs and reveal the dynamic evolution of cellular heterogeneity.

Duke Scholars

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Published In

Science advances

DOI

EISSN

2375-2548

ISSN

2375-2548

Publication Date

June 2020

Volume

6

Issue

24

Start / End Page

eaba4971

Related Subject Headings

  • Transcriptome
  • MicroRNAs
  • Gene Expression Regulation
  • Gene Expression Profiling
  • Biopsy
 

Citation

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Wang, Z., Qi, L., Yang, Y., Lu, M., Xie, K., Zhao, X., … Shi, P. (2020). High-throughput intracellular biopsy of microRNAs for dissecting the temporal dynamics of cellular heterogeneity. Science Advances, 6(24), eaba4971. https://doi.org/10.1126/sciadv.aba4971
Wang, Zixun, Lin Qi, Yang Yang, Mingxing Lu, Kai Xie, Xi Zhao, Elvis Hung Chi Cheung, et al. “High-throughput intracellular biopsy of microRNAs for dissecting the temporal dynamics of cellular heterogeneity.Science Advances 6, no. 24 (June 2020): eaba4971. https://doi.org/10.1126/sciadv.aba4971.
Wang Z, Qi L, Yang Y, Lu M, Xie K, Zhao X, et al. High-throughput intracellular biopsy of microRNAs for dissecting the temporal dynamics of cellular heterogeneity. Science advances. 2020 Jun;6(24):eaba4971.
Wang, Zixun, et al. “High-throughput intracellular biopsy of microRNAs for dissecting the temporal dynamics of cellular heterogeneity.Science Advances, vol. 6, no. 24, June 2020, p. eaba4971. Epmc, doi:10.1126/sciadv.aba4971.
Wang Z, Qi L, Yang Y, Lu M, Xie K, Zhao X, Cheung EHC, Wang Y, Jiang X, Zhang W, Huang L, Wang X, Shi P. High-throughput intracellular biopsy of microRNAs for dissecting the temporal dynamics of cellular heterogeneity. Science advances. 2020 Jun;6(24):eaba4971.

Published In

Science advances

DOI

EISSN

2375-2548

ISSN

2375-2548

Publication Date

June 2020

Volume

6

Issue

24

Start / End Page

eaba4971

Related Subject Headings

  • Transcriptome
  • MicroRNAs
  • Gene Expression Regulation
  • Gene Expression Profiling
  • Biopsy