Cold sensitivity of the SARS-CoV-2 spike ectodomain.

Journal Article (Journal Article)

The SARS-CoV-2 spike (S) protein, a primary target for COVID-19 vaccine development, presents its receptor binding domain in two conformations, the receptor-accessible 'up' or receptor-inaccessible 'down' states. Here we report that the commonly used stabilized S ectodomain construct '2P' is sensitive to cold temperatures, and this cold sensitivity is abrogated in a 'down' state-stabilized ectodomain. Our findings will impact structural, functional and vaccine studies that use the SARS-CoV-2 S ectodomain.

Full Text

Duke Authors

Cited Authors

  • Edwards, RJ; Mansouri, K; Stalls, V; Manne, K; Watts, B; Parks, R; Janowska, K; Gobeil, SMC; Kopp, M; Li, D; Lu, X; Mu, Z; Deyton, M; Oguin, TH; Sprenz, J; Williams, W; Saunders, KO; Montefiori, D; Sempowski, GD; Henderson, R; Munir Alam, S; Haynes, BF; Acharya, P

Published Date

  • February 2021

Published In

Volume / Issue

  • 28 / 2

Start / End Page

  • 128 - 131

PubMed ID

  • 33402708

Pubmed Central ID

  • PMC7878407

Electronic International Standard Serial Number (EISSN)

  • 1545-9985

Digital Object Identifier (DOI)

  • 10.1038/s41594-020-00547-5


  • eng

Conference Location

  • United States