An Improved Protocol for the Production of Lentiviral Vectors.
Journal Article (Journal Article)
Lentiviral vectors are an ideal gene-delivery system for large gene-editing tools, such as the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system, due to their high packaging capacity and broad tropism. Here, we present a calcium phosphate-based protocol for lentiviral production and concentration for in vitro and in vivo use. This revised procedure has been optimized to ensure high viral titers and transduction efficiency and is scalable to meet specific production needs.
Full Text
Duke Authors
Cited Authors
- Brown, LY; Dong, W; Kantor, B
Published Date
- December 18, 2020
Published In
Volume / Issue
- 1 / 3
Start / End Page
- 100152 -
PubMed ID
- 33377046
Pubmed Central ID
- PMC7757317
Electronic International Standard Serial Number (EISSN)
- 2666-1667
Digital Object Identifier (DOI)
- 10.1016/j.xpro.2020.100152
Language
- eng
Conference Location
- United States