An Improved Protocol for the Production of Lentiviral Vectors.

Journal Article (Journal Article)

Lentiviral vectors are an ideal gene-delivery system for large gene-editing tools, such as the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system, due to their high packaging capacity and broad tropism. Here, we present a calcium phosphate-based protocol for lentiviral production and concentration for in vitro and in vivo use. This revised procedure has been optimized to ensure high viral titers and transduction efficiency and is scalable to meet specific production needs.

Full Text

Duke Authors

Cited Authors

  • Brown, LY; Dong, W; Kantor, B

Published Date

  • December 18, 2020

Published In

Volume / Issue

  • 1 / 3

Start / End Page

  • 100152 -

PubMed ID

  • 33377046

Pubmed Central ID

  • PMC7757317

Electronic International Standard Serial Number (EISSN)

  • 2666-1667

Digital Object Identifier (DOI)

  • 10.1016/j.xpro.2020.100152

Language

  • eng

Conference Location

  • United States