Rapid and Efficient Production of Coronary Artery Ligation and Myocardial Infarction in Mice Using Surgical Clips.

Journal Article (Journal Article)

Aims

The coronary artery ligation model in rodents mimics human myocardial infarction (MI). Normally mechanical ventilation and prolonged anesthesia period are needed. Recently, a method has been developed to create MI by popping-out the heart (without ventilation) followed by immediate suture ligation. Mortality is high due to the time-consuming suture ligation process while the heart is exposed. We sought to improve this method and reduce mortality by rapid coronary ligation using a surgical clip instead of a suture.

Methods and results

Mice were randomized into 3 groups: clip MI (CMI), suture MI (SMI), or sham (SHAM). In all groups, heart was manually exposed without intubation through a small incision on the chest wall. Unlike the conventional SMI method, mice in the CMI group received a metal clip on left anterior descending artery (LAD), quickly dispensed by an AutoSuture Surgiclip™. The CMI method took only 1/3 of ligation time of the standard SMI method and improved post-MI survival rate. TTC staining and Masson's trichrome staining revealed a similar degree of infarct size in the SMI and CMI groups. Echocardiograph confirmed that both SMI and CMI groups had a similar reduction of ejection fraction and fraction shortening over the time. Histological analysis showed that the numbers of CD68+ macrophages and apoptotic cells (TUNEL-positive) are indistinguishable between the two groups.

Conclusion

This new method, taking only less than 3 minutes to complete, represents an efficient myocardial infarction model in rodents.

Full Text

Duke Authors

Cited Authors

  • Andrade, JNBMD; Tang, J; Hensley, MT; Vandergriff, A; Cores, J; Henry, E; Allen, TA; Caranasos, TG; Wang, Z; Zhang, T; Zhang, J; Cheng, K

Published Date

  • January 2015

Published In

Volume / Issue

  • 10 / 11

Start / End Page

  • e0143221 -

PubMed ID

  • 26599500

Pubmed Central ID

  • 26599500

Electronic International Standard Serial Number (EISSN)

  • 1932-6203

International Standard Serial Number (ISSN)

  • 1932-6203

Digital Object Identifier (DOI)

  • 10.1371/journal.pone.0143221

Language

  • eng