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Pannexin 1 channels control the hemodynamic response to hypoxia by regulating O2-sensitive extracellular ATP in blood.

Publication ,  Journal Article
Kirby, BS; Sparks, MA; Lazarowski, ER; Lopez Domowicz, DA; Zhu, H; McMahon, TJ
Published in: Am J Physiol Heart Circ Physiol
March 1, 2021

Pannexin 1 (Panx1) channels export ATP and may contribute to increased concentration of the vasodilator ATP in plasma during hypoxia in vivo. We hypothesized that Panx1 channels and associated ATP export contribute to hypoxic vasodilation, a mechanism that facilitates the matching of oxygen delivery to metabolic demand of tissue. Male and female mice devoid of Panx1 (Panx1-/-) and wild-type controls (WT) were anesthetized, mechanically ventilated, and instrumented with a carotid artery catheter or femoral artery flow transducer for hemodynamic and plasma ATP monitoring during inhalation of 21% (normoxia) or 10% oxygen (hypoxia). ATP export from WT vs. Panx1-/-erythrocytes (RBC) was determined ex vivo via tonometer experimentation across progressive deoxygenation. Mean arterial pressure (MAP) was similar in Panx1-/- (n = 6) and WT (n = 6) mice in normoxia, but the decrease in MAP in hypoxia seen in WT was attenuated in Panx1-/- mice (-16 ± 9% vs. -2 ± 8%; P < 0.05). Hindlimb blood flow (HBF) was significantly lower in Panx1-/- (n = 6) vs. WT (n = 6) basally, and increased in WT but not Panx1-/- mice during hypoxia (8 ± 6% vs. -10 ± 13%; P < 0.05). Estimation of hindlimb vascular conductance using data from the MAP and HBF experiments showed an average response of 28% for WT vs. -9% for Panx1-/- mice. Mean venous plasma ATP during hypoxia was 57% lower in Panx1-/- (n = 6) vs. WT mice (n = 6; P < 0.05). Mean hypoxia-induced ATP export from RBCs from Panx1-/- mice (n = 8) was 82% lower than that from WT (n = 8; P < 0.05). Panx1 channels participate in hemodynamic responses consistent with hypoxic vasodilation by regulating hypoxia-sensitive extracellular ATP levels in blood.NEW & NOTEWORTHY Export of vasodilator ATP from red blood cells requires pannexin 1. Blood plasma ATP elevations in response to hypoxia in mice require pannexin 1. Hemodynamic responses to hypoxia are accompanied by increased plasma ATP in mice in vivo and require pannexin 1.

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Published In

Am J Physiol Heart Circ Physiol

DOI

EISSN

1522-1539

Publication Date

March 1, 2021

Volume

320

Issue

3

Start / End Page

H1055 / H1065

Location

United States

Related Subject Headings

  • Vasodilation
  • Regional Blood Flow
  • Oxygen
  • Nerve Tissue Proteins
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Hypoxia
  • Hypotension
 

Citation

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Kirby, B. S., Sparks, M. A., Lazarowski, E. R., Lopez Domowicz, D. A., Zhu, H., & McMahon, T. J. (2021). Pannexin 1 channels control the hemodynamic response to hypoxia by regulating O2-sensitive extracellular ATP in blood. Am J Physiol Heart Circ Physiol, 320(3), H1055–H1065. https://doi.org/10.1152/ajpheart.00651.2020
Kirby, Brett S., Matthew A. Sparks, Eduardo R. Lazarowski, Denise A. Lopez Domowicz, Hongmei Zhu, and Timothy J. McMahon. “Pannexin 1 channels control the hemodynamic response to hypoxia by regulating O2-sensitive extracellular ATP in blood.Am J Physiol Heart Circ Physiol 320, no. 3 (March 1, 2021): H1055–65. https://doi.org/10.1152/ajpheart.00651.2020.
Kirby BS, Sparks MA, Lazarowski ER, Lopez Domowicz DA, Zhu H, McMahon TJ. Pannexin 1 channels control the hemodynamic response to hypoxia by regulating O2-sensitive extracellular ATP in blood. Am J Physiol Heart Circ Physiol. 2021 Mar 1;320(3):H1055–65.
Kirby, Brett S., et al. “Pannexin 1 channels control the hemodynamic response to hypoxia by regulating O2-sensitive extracellular ATP in blood.Am J Physiol Heart Circ Physiol, vol. 320, no. 3, Mar. 2021, pp. H1055–65. Pubmed, doi:10.1152/ajpheart.00651.2020.
Kirby BS, Sparks MA, Lazarowski ER, Lopez Domowicz DA, Zhu H, McMahon TJ. Pannexin 1 channels control the hemodynamic response to hypoxia by regulating O2-sensitive extracellular ATP in blood. Am J Physiol Heart Circ Physiol. 2021 Mar 1;320(3):H1055–H1065.

Published In

Am J Physiol Heart Circ Physiol

DOI

EISSN

1522-1539

Publication Date

March 1, 2021

Volume

320

Issue

3

Start / End Page

H1055 / H1065

Location

United States

Related Subject Headings

  • Vasodilation
  • Regional Blood Flow
  • Oxygen
  • Nerve Tissue Proteins
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Hypoxia
  • Hypotension