Liquid-culture protocols for synchronous starvation, growth, dauer formation, and dietary restriction of Caenorhabditis elegans .

Journal Article (Journal Article)

Standard laboratory culture of Caenorhabditis elegans utilizes solid growth media with a bacterial food source. However, this culture method limits control of food availability and worm population density, factors that impact many life-history traits. Here, we describe liquid-culture protocols for precisely modulating bacterial food availability and population density, facilitating reliable production of arrested L1 larvae, dauer larvae, dietarily restricted worms, or well-fed worms. Worms can be grown in small quantities for standard assays or in the millions for other applications. For complete details on the use and execution of these protocols, please refer to Hibshman et al. (2016), Webster et al. (2018), and Jordan et al. (2019).

Full Text

Duke Authors

Cited Authors

  • Hibshman, JD; Webster, AK; Baugh, LR

Published Date

  • March 2021

Published In

Volume / Issue

  • 2 / 1

Start / End Page

  • 100276 -

PubMed ID

  • 33490989

Pubmed Central ID

  • 33490989

Electronic International Standard Serial Number (EISSN)

  • 2666-1667

International Standard Serial Number (ISSN)

  • 2666-1667

Digital Object Identifier (DOI)

  • 10.1016/j.xpro.2020.100276

Language

  • eng