Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets.

Journal Article (Journal Article)

Cells, the basic units of biological structure and function, vary broadly in type and state. Single-cell genomics can characterize cell identity and function, but limitations of ease and scale have prevented its broad application. Here we describe Drop-seq, a strategy for quickly profiling thousands of individual cells by separating them into nanoliter-sized aqueous droplets, associating a different barcode with each cell's RNAs, and sequencing them all together. Drop-seq analyzes mRNA transcripts from thousands of individual cells simultaneously while remembering transcripts' cell of origin. We analyzed transcriptomes from 44,808 mouse retinal cells and identified 39 transcriptionally distinct cell populations, creating a molecular atlas of gene expression for known retinal cell classes and novel candidate cell subtypes. Drop-seq will accelerate biological discovery by enabling routine transcriptional profiling at single-cell resolution. VIDEO ABSTRACT.

Full Text

Duke Authors

Cited Authors

  • Macosko, EZ; Basu, A; Satija, R; Nemesh, J; Shekhar, K; Goldman, M; Tirosh, I; Bialas, AR; Kamitaki, N; Martersteck, EM; Trombetta, JJ; Weitz, DA; Sanes, JR; Shalek, AK; Regev, A; McCarroll, SA

Published Date

  • May 21, 2015

Published In

Volume / Issue

  • 161 / 5

Start / End Page

  • 1202 - 1214

PubMed ID

  • 26000488

Pubmed Central ID

  • PMC4481139

Electronic International Standard Serial Number (EISSN)

  • 1097-4172

Digital Object Identifier (DOI)

  • 10.1016/j.cell.2015.05.002

Language

  • eng

Conference Location

  • United States