The Kalanchoë genome provides insights into convergent evolution and building blocks of crassulacean acid metabolism.

Journal Article (Journal Article)

Crassulacean acid metabolism (CAM) is a water-use efficient adaptation of photosynthesis that has evolved independently many times in diverse lineages of flowering plants. We hypothesize that convergent evolution of protein sequence and temporal gene expression underpins the independent emergences of CAM from C3 photosynthesis. To test this hypothesis, we generate a de novo genome assembly and genome-wide transcript expression data for Kalanchoë fedtschenkoi, an obligate CAM species within the core eudicots with a relatively small genome (~260 Mb). Our comparative analyses identify signatures of convergence in protein sequence and re-scheduling of diel transcript expression of genes involved in nocturnal CO2 fixation, stomatal movement, heat tolerance, circadian clock, and carbohydrate metabolism in K. fedtschenkoi and other CAM species in comparison with non-CAM species. These findings provide new insights into molecular convergence and building blocks of CAM and will facilitate CAM-into-C3 photosynthesis engineering to enhance water-use efficiency in crops.

Full Text

Duke Authors

Cited Authors

  • Yang, X; Hu, R; Yin, H; Jenkins, J; Shu, S; Tang, H; Liu, D; Weighill, DA; Cheol Yim, W; Ha, J; Heyduk, K; Goodstein, DM; Guo, H-B; Moseley, RC; Fitzek, E; Jawdy, S; Zhang, Z; Xie, M; Hartwell, J; Grimwood, J; Abraham, PE; Mewalal, R; Beltrán, JD; Boxall, SF; Dever, LV; Palla, KJ; Albion, R; Garcia, T; Mayer, JA; Don Lim, S; Man Wai, C; Peluso, P; Van Buren, R; De Paoli, HC; Borland, AM; Guo, H; Chen, J-G; Muchero, W; Yin, Y; Jacobson, DA; Tschaplinski, TJ; Hettich, RL; Ming, R; Winter, K; Leebens-Mack, JH; Smith, JAC; Cushman, JC; Schmutz, J; Tuskan, GA

Published Date

  • December 2017

Published In

Volume / Issue

  • 8 / 1

Start / End Page

  • 1899 -

PubMed ID

  • 29196618

Pubmed Central ID

  • PMC5711932

Electronic International Standard Serial Number (EISSN)

  • 2041-1723

International Standard Serial Number (ISSN)

  • 2041-1723

Digital Object Identifier (DOI)

  • 10.1038/s41467-017-01491-7

Language

  • eng