Nucleo-cytoplasmic Partitioning of ARF Proteins Controls Auxin Responses in Arabidopsis thaliana.

Journal Article (Journal Article)

The phytohormone auxin plays crucial roles in nearly every aspect of plant growth and development. The auxin response factor (ARF) transcription factor family regulates auxin-responsive gene expression and exhibits nuclear localization in regions of high auxin responsiveness. Here we show that the ARF7 and ARF19 proteins accumulate in micron-sized assemblies within the cytoplasm of tissues with attenuated auxin responsiveness. We found that the intrinsically disordered middle region and the folded PB1 interaction domain of ARFs drive protein assembly formation. Mutation of a single lysine within the PB1 domain abrogates cytoplasmic assemblies, promotes ARF nuclear localization, and results in an altered transcriptome and morphological defects. Our data suggest a model in which ARF nucleo-cytoplasmic partitioning regulates auxin responsiveness, providing a mechanism for cellular competence for auxin signaling.

Full Text

Duke Authors

Cited Authors

  • Powers, SK; Holehouse, AS; Korasick, DA; Schreiber, KH; Clark, NM; Jing, H; Emenecker, R; Han, S; Tycksen, E; Hwang, I; Sozzani, R; Jez, JM; Pappu, RV; Strader, LC

Published Date

  • October 2019

Published In

Volume / Issue

  • 76 / 1

Start / End Page

  • 177 - 190.e5

PubMed ID

  • 31421981

Pubmed Central ID

  • 31421981

Electronic International Standard Serial Number (EISSN)

  • 1097-4164

International Standard Serial Number (ISSN)

  • 1097-2765

Digital Object Identifier (DOI)

  • 10.1016/j.molcel.2019.06.044

Language

  • eng